Recombinant Escherichia coli Acetate CoA-transferase subunit alpha (atoD), Escherichia coli (strain K12), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 30.5kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Escherichia coli Acetate CoA-transferase subunit alpha (atoD)

Recombinant Escherichia coli Acetate CoA-transferase subunit alpha (atoD) — Coenzyme A transferase which is involved in short-chain fatty acid degradation and catalyzes the activation of short-chain fatty acids to their respective CoA thiolesters. Purity >90%.

SKU: BCRECP-002161 Categories: ,
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Product Specifications

Product SkuBCRECP-002161
Product DescriptionRecombinant Escherichia coli Acetate CoA-transferase subunit alpha (atoD) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-220aa. [Accession | P76458].
Uniprot No.P76458
Gene NamesatoD
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-220aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight30.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Coenzyme A transferase which is involved in short-chain fatty acid degradation and catalyzes the activation of short-chain fatty acids to their respective CoA thiolesters. During acetoacetate degradation, catalyzes the transfer of CoA from acetyl-CoA to acetoacetate by a mechanism involving a covalent enzyme-CoA compound as a reaction intermediate. Utilizes a variety of short chain acyl-CoA and carboxylic acid substrates but exhibits maximal activity with normal and 3-keto substrates.

Biological Context

Subcellular Location: Cytoplasm
Pathway: Lipid metabolism; short-chain fatty acid metabolism

Product Specifications

Recombinant Escherichia coli Acetate CoA-transferase subunit alpha (atoD) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 1-220aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 30.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Coenzyme A transferase which is involved in short-chain fatty acid degradation and catalyzes the activation of short-chain fatty acids to their respective CoA thiolesters (PubMed:1103739, PubMed:3025185). During acetoacetate degradation, catalyzes the transfer of CoA from acetyl-CoA to acetoacetate by a mechanism involving a covalent enzyme-CoA compound as a reaction intermediate (PubMed:1103741). Utilizes a variety of short chain acyl-CoA and carboxylic acid substrates but exhibits maximal activity with normal and 3-keto substrates (PubMed:1103739)

Subcellular Location

Cytoplasm

Subunit

Heterotetramer composed of two alpha subunits (AtoD) and two beta subunits (AtoA)

Pathway

Lipid metabolism; short-chain fatty acid metabolism

Gene: atoD  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Acetoacetyl-CoA transferase subunit alpha; Acetyl-CoA:acetoacetyl-CoA transferase subunit alpha
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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