Recombinant Escherichia coli Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (bioA), Escherichia coli (strain K12), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 54.8kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Escherichia coli Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (bioA)

Recombinant Escherichia coli Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (bioA) — Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). Purity >90%.

SKU: BCRECP-002197 Categories: ,
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Product Specifications

Product SkuBCRECP-002197
Product DescriptionRecombinant Escherichia coli Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (bioA) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-429aa. [Accession | P12995].
Uniprot No.P12995
Gene NamesbioA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-429aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight54.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only animotransferase known to utilize SAM as an amino donor. Complements a bioU deletion in Synechocystis PCC 6803.

Biological Context

Subcellular Location: Cytoplasm
Pathway: Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1

Product Specifications

Recombinant Escherichia coli Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (bioA) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 1-429aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 54.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA) (PubMed:1092681, PubMed:1092682). It is the only animotransferase known to utilize SAM as an amino donor (PubMed:1092681, PubMed:1092682). Complements a bioU deletion in Synechocystis PCC 6803 (PubMed:32042199)

Subcellular Location

Cytoplasm

Subunit

Homodimer

Pathway

Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1

Gene: bioA  |  Organism: Escherichia coli (strain K12)  |  Synonyms: 7,8-diamino-pelargonic acid aminotransferase; 7,8-diaminononanoate synthase; Diaminopelargonic acid synthase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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