Recombinant Escherichia coli Adenylate cyclase (cyaA) Protein, Escherichia coli (strain K12), in vitro E.coli expression system, N-terminal 6xHis-tagged, 99.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli Adenylate cyclase (cyaA) Protein

Recombinant Escherichia coli Adenylate cyclase (cyaA) Protein — Catalyzes the formation of the second messenger cAMP from ATP. Purity >90%.

SKU: BCRECP-00677TM Category:
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Product Specifications

Uniprot No.P00936
Gene NamescyaA
PurityGreater than 90% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-848aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-tagged
Molecular weight99.9kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Catalyzes the formation of the second messenger cAMP from ATP. Its transcript is probably degraded by endoribonuclease LS (rnlA), decreasing cAMP levels and the negative regulator Crp-cAMP, which then induces its own transcription again.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Escherichia coli Adenylate cyclase (cyaA) Protein is a recombinant protein from Escherichia coli (strain K12), expressed in in vitro E.coli expression system, covering amino acids 1-848aa, with N-terminal 6xHis-tagged tag, molecular weight 99.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Not implied unless activity data are explicitly listed on this product page, the datasheet, or the CoA.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Catalyzes the formation of the second messenger cAMP from ATP. Its transcript is probably degraded by endoribonuclease LS (rnlA), decreasing cAMP levels and the negative regulator Crp-cAMP, which then induces its own transcription again

Subcellular Location

Cytoplasm

Gene: cyaA  |  Organism: Escherichia coli (strain K12)  |  Synonyms: ATP pyrophosphate-lyase; Adenylyl cyclase
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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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