Recombinant Escherichia coli Alpha-ketoglutarate-dependent dioxygenase AlkB (alkB) — Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Purity >90%.
| Product Sku | BCRECP-002070 |
| Product Description | Recombinant Escherichia coli Alpha-ketoglutarate-dependent dioxygenase AlkB (alkB) Protein is expressed from E.coli with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. It contains 1-216aa. [Accession | P05050]. |
| Uniprot No. | P05050 |
| Gene Names | alkB |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 1-216aa |
| Species | Escherichia coli (strain K12) |
| Tag Info | N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged |
| Molecular weight | 44.1kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Epigenetics and Nuclear Signaling |
Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine.
Recombinant Escherichia coli Alpha-ketoglutarate-dependent dioxygenase AlkB (alkB) — Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Purity >90%. Suitable for ELISA and Western Blot applications.
Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.
Biological Activity: Please contact us to obtain bioactivity data.
Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.
Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents)
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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.
Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.
Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.
