Recombinant Escherichia coli Antitoxin CcdA (ccdA), Escherichia coli, E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 15.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli Antitoxin CcdA (ccdA)

Recombinant Escherichia coli Antitoxin CcdA (ccdA) — Antitoxin component of a type II toxin-antitoxin (TA) system which inhibits the post-segregational killing (PSK) of plasmid-free cells, also referred to as a plasmid addiction system. Purity >90%.

SKU: BCRECP-002303 Category:
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Product Specifications

Product SkuBCRECP-002303
Product DescriptionRecombinant Escherichia coli Antitoxin CcdA (ccdA) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-72aa. [Accession | Q46995].
Uniprot No.Q46995
Gene NamesccdA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-72aa
SpeciesEscherichia coli
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight15.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Antitoxin component of a type II toxin-antitoxin (TA) system which inhibits the post-segregational killing (PSK) of plasmid-free cells, also referred to as a plasmid addiction system. Binds to and blocks the activity of CcdB; will also remove bound CcdB protein from the CcdB-GyrA complex by forming a CcdA-CcdB complex, a process termed rejuvenation. Functions as a transcriptional corepressor for the ccdAB operon, repression also requires CcdB.

Product Specifications

Recombinant Escherichia coli Antitoxin CcdA (ccdA) is a recombinant protein from Escherichia coli, expressed in E.coli, covering amino acids 1-72aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 15.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Antitoxin component of a type II toxin-antitoxin (TA) system which inhibits the post-segregational killing (PSK) of plasmid-free cells, also referred to as a plasmid addiction system. Binds to and blocks the activity of CcdB; will also remove bound CcdB protein from the CcdB-GyrA complex by forming a CcdA-CcdB complex, a process termed rejuvenation. Functions as a transcriptional corepressor for the ccdAB operon, repression also requires CcdB (By similarity)

Subunit

Homodimer in solution and when bound to DNA. Forms a complex with toxin CcdB; the CcdA-CcdB(2) trimer is sufficient for rejuvenation, whereas maximal operon repression occurs with CcdA(2)CcdB(2) (By similarity)

Gene: ccdA  |  Organism: Escherichia coli  |  Synonyms: Protein LetA

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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