Recombinant Escherichia coli Crossover junction endodeoxyribonuclease RuvC (ruvC), Escherichia coli (strain K12), E.coli, N-terminal 6xHis-SUMO-tagged, 34.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli Crossover junction endodeoxyribonuclease RuvC (ruvC)

Recombinant Escherichia coli Crossover junction endodeoxyribonuclease RuvC (ruvC) — An in vitro resolvase system that forms and processes HJ has been reconstituted with DNA substrates, RuvA, RuvB and RuvC. Purity >90%.

SKU: BCRECP-004307 Category:
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Product Specifications

Product SkuBCRECP-004307
Product DescriptionRecombinant Escherichia coli Crossover junction endodeoxyribonuclease RuvC (ruvC) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 2-173aa. [Accession | P0A814].
Uniprot No.P0A814
Gene NamesruvC
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-173aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight34.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

An in vitro resolvase system that forms and processes HJ has been reconstituted with DNA substrates, RuvA, RuvB and RuvC. RuvA-RuvB increases the rate of strand exchange (branch migration), dissociates the RecA filament and allows RuvC to cleave in both orientations at the cruciform junction. HJ-RuvA-RuvB-RuvC complexes resolve Holliday junctions and also undergo branch migration, providing evidence for a coupled branch migration/HJ resolution reaction.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Escherichia coli Crossover junction endodeoxyribonuclease RuvC (ruvC) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 2-173aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 34.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

An in vitro resolvase system that forms and processes HJ has been reconstituted with DNA substrates, RuvA, RuvB and RuvC. RuvA-RuvB increases the rate of strand exchange (branch migration), dissociates the RecA filament and allows RuvC to cleave in both orientations at the cruciform junction (PubMed:10421637, PubMed:9160752). HJ-RuvA-RuvB-RuvC complexes resolve Holliday junctions and also undergo branch migration, providing evidence for a coupled branch migration/HJ resolution reaction (PubMed:10421637)

Subcellular Location

Cytoplasm

Subunit

Homodimer which binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ (By similarity). A mix of monomer and homodimer in solution (PubMed:8106500). Homodimer which binds DNA; there is probably a monomer-dimer equilibrium during DNA-binding (PubMed:10471285, PubMed:1661673, PubMed:7638215, PubMed:7923356, PubMed:9135161). Can form complexes with RuvA and Holliday junction (HJ) DNA, in which the resolvase activity is inhibited (PubMed:9000618). Forms a complex with RuvB without DNA; RuvA-RuvC complexes were not detected in the absence of DNA (PubMed:9160752). In the presence of HJ DNA a DNA-RuvA-RuvB-RuvC complex forms with 2 rings of RuvB that resolves upon addition of ATP. Upon resolution the protein complex dissociates from DNA (PubMed:10421637, PubMed:9637927)

Gene: ruvC  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Holliday junction endonuclease RuvC; Holliday junction nuclease RuvC; Holliday junction resolvase RuvC
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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