Recombinant Escherichia coli DNA helicase II (uvrD) — A helicase with DNA-dependent ATPase activity. Purity >85%.
| Product Sku | BCRECP-004697 |
| Product Description | Recombinant Escherichia coli DNA helicase II (uvrD) Protein is expressed from E.coli with Tag-Free. It contains 1-720aa. [Accession | P03018]. |
| Uniprot No. | P03018 |
| Gene Names | uvrD |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 1-720aa |
| Species | Escherichia coli (strain K12) |
| Tag Info | Tag-Free |
| Molecular weight | 82.0kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Others |
A helicase with DNA-dependent ATPase activity. Unwinds DNA duplexes with 3'-5' polarity. Translocates on single-stranded DNA with 3'-5' polarity.
Recombinant Escherichia coli DNA helicase II (uvrD) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 1-720aa, with Tag-Free tag, molecular weight 82.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.
Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.
Biological Activity: Please contact us to obtain bioactivity data.
Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.
A helicase with DNA-dependent ATPase activity (PubMed:6319401, PubMed:8419285). Unwinds DNA duplexes with 3'-5' polarity (PubMed:2170128, PubMed:8419285). Translocates on single-stranded DNA with 3'-5' polarity (PubMed:2942537). Initiates unwinding more efficiently from a nicked substrate than double-stranded DNA (PubMed:2170128, PubMed:8419285). Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair, and probably also in repair of alkylated DNA (Probable) (PubMed:25484163)
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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.
Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.
Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.
