Recombinant Escherichia coli Hemolysin E, chromosomal (hlyE), partial

Recombinant Escherichia coli Hemolysin E, chromosomal (hlyE), partial — Toxin, which has some hemolytic activity towards mammalian cells. Purity >90%.

SKU: BCRECP-002995 Category:

Product Specifications

Product SkuBCRECP-002995
Product DescriptionRecombinant Escherichia coli Hemolysin E, chromosomal (hlyE) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 2-182aa. [Accession | P77335].
Uniprot No.P77335
Gene NameshlyE
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-182aa
SpeciesEscherichia coli (strain K12)
Tag InfoC-terminal 6xHis-tagged
Molecular weight21.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Toxin, which has some hemolytic activity towards mammalian cells. Acts by forming a pore-like structure upon contact with mammalian cells.

Biological Context

Subcellular Location: Secreted; Periplasm; Host cell membrane (Single-pass membrane protein)

Product Specifications

Recombinant Escherichia coli Hemolysin E, chromosomal (hlyE), partial is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 2-182aa, with C-terminal 6xHis-tagged tag, molecular weight 21.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Toxin, which has some hemolytic activity towards mammalian cells. Acts by forming a pore-like structure upon contact with mammalian cells

Subcellular Location

Secreted; Periplasm; Host cell membrane (Single-pass membrane protein)

Subunit

Monomer and oligomer. In periplasm, it is present as a monomer, while in outer membrane vesicles, it oligomerizes to form a pore structure that is active. The pore is formed by a dodecamer

Gene: hlyE  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Cytotoxin ClyA; Hemolysis-inducing protein; Latent pore-forming 34 kDa hemolysin; Silent hemolysin SheA
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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