Recombinant Escherichia coli Lactose permease (lacY), partial Protein, Escherichia coli (strain K12), in vitro E.coli expression system, N-terminal 10xHis-tagged, 34.4kDa, purity >85% as determined by SDS-PAGE.

Recombinant Escherichia coli Lactose permease (lacY), partial Protein

Recombinant Escherichia coli Lactose permease (lacY), partial Protein — Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system). Purity >85%.

SKU: BCRECP-00047TM Category:
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Product Specifications

Uniprot No.P02920
Gene NameslacY
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-250aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 10xHis-tagged
Molecular weight34.4kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system). Can transport lactose, melibiose, the synthetic disaccharide lactulose or the analog methyl-1-thio-beta,D-galactopyranoside (TMG), but not sucrose or fructose. The substrate specificity is directed toward the galactopyranosyl moiety of the substrate.

Biological Context

Subcellular Location: Cell inner membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Escherichia coli Lactose permease (lacY), partial Protein is a recombinant protein from Escherichia coli (strain K12), expressed in in vitro E.coli expression system, covering amino acids 1-250aa, with N-terminal 10xHis-tagged tag, molecular weight 34.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Not implied unless activity data are explicitly listed on this product page, the datasheet, or the CoA.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system). Can transport lactose, melibiose, the synthetic disaccharide lactulose or the analog methyl-1-thio-beta,D-galactopyranoside (TMG), but not sucrose or fructose (PubMed:18177889, PubMed:1848449, PubMed:22106930, PubMed:7000781, PubMed:7028742). The substrate specificity is directed toward the galactopyranosyl moiety of the substrate (PubMed:22106930)

Subcellular Location

Cell inner membrane (Multi-pass membrane protein)

Subunit

Monomer

Gene: lacY  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Lactose-proton symport
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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