Recombinant Escherichia coli Lactose permease (lacY), partial Protein, Escherichia coli (strain K12), in vitro E.coli expression system, N-terminal 10xHis-tagged, 34.4kDa, purity >85% as determined by SDS-PAGE.

Recombinant Escherichia coli Lactose permease (lacY), partial Protein

Recombinant Escherichia coli Lactose permease (lacY), partial Protein — Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system). Purity >85%.

SKU: BCRECP-00047TM Category:
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Product Specifications

Uniprot No.P02920
Gene NameslacY
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-250aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 10xHis-tagged
Molecular weight34.4kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system). Can transport lactose, melibiose, the synthetic disaccharide lactulose or the analog methyl-1-thio-beta,D-galactopyranoside (TMG), but not sucrose or fructose. The substrate specificity is directed toward the galactopyranosyl moiety of the substrate.

Biological Context

Subcellular Location: Cell inner membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Escherichia coli Lactose permease (lacY), partial Protein is a recombinant protein from Escherichia coli (strain K12), expressed in in vitro E.coli expression system, covering amino acids 1-250aa, with N-terminal 10xHis-tagged tag, molecular weight 34.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system). Can transport lactose, melibiose, the synthetic disaccharide lactulose or the analog methyl-1-thio-beta,D-galactopyranoside (TMG), but not sucrose or fructose (PubMed:18177889, PubMed:1848449, PubMed:22106930, PubMed:7000781, PubMed:7028742). The substrate specificity is directed toward the galactopyranosyl moiety of the substrate (PubMed:22106930)

Subcellular Location

Cell inner membrane (Multi-pass membrane protein)

Subunit

Monomer

Gene: lacY  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Lactose-proton symport
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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