Recombinant Escherichia coli Lipopolysaccharide assembly protein B (lapB), partial, Escherichia coli (strain K12), E.coli, N-terminal 6xHis-SUMO-tagged, 58.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli Lipopolysaccharide assembly protein B (lapB), partial

Recombinant Escherichia coli Lipopolysaccharide assembly protein B (lapB), partial — Modulates cellular lipopolysaccharide (LPS) levels by regulating LpxC, which is involved in lipid A biosynthesis. Purity >90%.

SKU: BCRECP-003240 Category:
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Product Specifications

Product SkuBCRECP-003240
Product DescriptionRecombinant Escherichia coli Lipopolysaccharide assembly protein B (lapB) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 17-389aa. [Accession | P0AB58].
Uniprot No.P0AB58
Gene NameslapB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region17-389aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight58.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Modulates cellular lipopolysaccharide (LPS) levels by regulating LpxC, which is involved in lipid A biosynthesis. Regulates the proteolytic activity of FtsH towards LpxC by acting as an adapter protein which interacts with FtsH via its transmembrane helices while its cytoplasmic domain recruits LpxC for degradation by FtsH. May also coordinate assembly of proteins involved in LPS synthesis at the plasma membrane.

Biological Context

Subcellular Location: Cell inner membrane (Single-pass membrane protein)

Product Specifications

Recombinant Escherichia coli Lipopolysaccharide assembly protein B (lapB), partial is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 17-389aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 58.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Modulates cellular lipopolysaccharide (LPS) levels by regulating LpxC, which is involved in lipid A biosynthesis. Regulates the proteolytic activity of FtsH towards LpxC by acting as an adapter protein which interacts with FtsH via its transmembrane helices while its cytoplasmic domain recruits LpxC for degradation by FtsH. May also coordinate assembly of proteins involved in LPS synthesis at the plasma membrane

Subcellular Location

Cell inner membrane (Single-pass membrane protein)

Subunit

Forms a heterotetramer with PbgA/YjeM consisting of 2 molecules of LapB and 2 molecules of PbgA; the complex dissociates when excess lipopolysaccharide (LPS) accumulates in the inner membrane due to overlapping binding sites for LPS and LapB on PbgA (PubMed:32430473, PubMed:32788728, PubMed:33260377, PubMed:35931690). Interacts with FtsH; the interaction promotes FtsH-mediated proteolysis of LpxC (PubMed:35931690)

Gene: lapB  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Lipopolysaccharide regulatory protein; LpxC degradation adapter protein B
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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