Recombinant Escherichia coli Minor curlin subunit (csgB), Biotinylated, Escherichia coli (strain K12), E.coli, N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged, 61.5kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Escherichia coli Minor curlin subunit (csgB), Biotinylated

Recombinant Escherichia coli Minor curlin subunit (csgB), Biotinylated — Curlin is the structural subunit of the curli fimbriae. Purity >90%.

SKU: BCRECP-002454 Categories: ,
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Product Specifications

Product SkuBCRECP-002454
Product DescriptionRecombinant Escherichia coli Minor curlin subunit (csgB) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. It contains 22-151aa. [Accession | P0ABK7].
Uniprot No.P0ABK7
Gene NamescsgB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region22-151aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Molecular weight61.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Curlin is the structural subunit of the curli fimbriae. Curli are coiled surface structures that assemble preferentially at growth temperatures below 37 degrees Celsius. Curli can bind to fibronectin.

Biological Context

Subcellular Location: Fimbrium

Product Specifications

Recombinant Escherichia coli Minor curlin subunit (csgB), Biotinylated is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 22-151aa, with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged tag, molecular weight 61.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Curlin is the structural subunit of the curli fimbriae. Curli are coiled surface structures that assemble preferentially at growth temperatures below 37 degrees Celsius. Curli can bind to fibronectin. The minor subunit is the nucleation component of curlin monomers. Coexpression of cellulose and thin aggregative fimbriae (curli fimbrae or fibers) leads to a hydrophobic network with tightly packed cells embedded in a highly inert matrix that confers cohesion, elasticity and tissue-like properties to colonies (PubMed:24097954)

Subcellular Location

Fimbrium

Gene: csgB  |  Organism: Escherichia coli (strain K12)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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