Recombinant Escherichia coli O127:H6 Intimin (eae), partial — An inverse autotransporter. Purity >90%.
| Product Sku | BCRECP-002576 |
| Product Description | Recombinant Escherichia coli O127:H6 Intimin (eae) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 658-939aa. [Accession | P19809]. |
| Uniprot No. | P19809 |
| Gene Names | eae |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 658-939aa |
| Species | Escherichia coli O127:H6 (strain E2348/69 / EPEC) |
| Tag Info | C-terminal 6xHis-tagged |
| Molecular weight | 37.0kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Others |
An inverse autotransporter. Adhesin, which mediates attachment to the human intestine epithelial cells.
Recombinant Escherichia coli O127:H6 Intimin (eae), partial — An inverse autotransporter. Purity >90%. Suitable for ELISA and Western Blot applications.
Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.
Biological Activity: Please contact us to obtain bioactivity data.
Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.
An inverse autotransporter (PubMed:25353290, PubMed:33277518). Adhesin, which mediates attachment to the human intestine epithelial cells (PubMed:2172966). Necessary for the production of attaching and effacing lesions on infected human tissue culture cells (PubMed:2172966, PubMed:10835344). Anchored to the outer membrane by binding to peptidoglycan (PGN) via its periplasmic domain, thus helping in receptor interactions during host invasion (Probable). PGN-binding may also aid in resisting mechanical and chemical stress during transit of the bacterium through the gastrointestinal tract of the host (Probable). Periplasmic domain binds purified E.coli PGN sacculi under acidic conditions in vitro and in vivo, but does not bind to chitin (PubMed:25353290). Periplasmic domain binds PGN sacculi with an apparent dissociation constant (Kd) of 0.8 uM (PubMed:25353290). No binding to PGN in vitro at normal physiological pH 7.4 (PubMed:25353290)
Cell outer membrane
Homodimer (PubMed:25353290). Interacts with Tir (PubMed:10835344, PubMed:10890451)
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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.
Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.
Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.
