Recombinant Escherichia coli O157:H7 Metalloprotease stcE (stcE), partial, Escherichia coli O157:H7, E.coli, N-terminal 6xHis-tagged, 32.3kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Escherichia coli O157:H7 Metalloprotease stcE (stcE), partial

Recombinant Escherichia coli O157:H7 Metalloprotease stcE (stcE), partial — Virulence factor that contributes to intimate adherence of enterohemorrhagic E. Purity >85%.

SKU: BCRECP-004495 Categories: , ,
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Product Specifications

Product SkuBCRECP-004495
Product DescriptionRecombinant Escherichia coli O157:H7 Metalloprotease stcE (stcE) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 296-551aa. [Accession | O82882].
Uniprot No.O82882
Gene NamesstcE
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region296-551aa
SpeciesEscherichia coli O157:H7
Tag InfoN-terminal 6xHis-tagged
Molecular weight32.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areasothers

Function

Virulence factor that contributes to intimate adherence of enterohemorrhagic E.coli (EHEC) O157:H7 to host cells. Is able to cleave the secreted human mucin 7 (MUC7) and the glycoprotein 340 (DMBT1/GP340). Also cleaves human C1 inhibitor (SERPING1), a regulator of multiple inflammatory pathways, and binds and localizes it to bacterial and host cell surfaces, protecting them from complement-mediated lysis.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Escherichia coli O157:H7 Metalloprotease stcE (stcE), partial is a recombinant protein from Escherichia coli O157:H7, expressed in E.coli, covering amino acids 296-551aa, with N-terminal 6xHis-tagged tag, molecular weight 32.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Virulence factor that contributes to intimate adherence of enterohemorrhagic E.coli (EHEC) O157:H7 to host cells. Is able to cleave the secreted human mucin 7 (MUC7) and the glycoprotein 340 (DMBT1/GP340). Also cleaves human C1 inhibitor (SERPING1), a regulator of multiple inflammatory pathways, and binds and localizes it to bacterial and host cell surfaces, protecting them from complement-mediated lysis. Therefore, the current model proposes two roles for StcE during infection: it acts first as a mucinase, allowing passage of EHEC through the oral cavity by cleaving the salivary glycoproteins that are responsible for bacterial aggregation. Similarly, in the colon, StcE cleaves the glycoproteins that protect the intestinal epithelial surface, allowing EHEC to come into close contact with host cell membranes. Secondly, it acts as an anti-inflammatory agent by localizing SERPING1 to cell membranes

Subcellular Location

Secreted

Gene: stcE  |  Organism: Escherichia coli O157:H7  |  Synonyms: Mucinase; Neutral zinc metalloprotease StcE; Secreted protease of C1 esterase inhibitor from EHEC
Key Publications

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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