Recombinant Escherichia coli O6:H1 Sensor protein PhoQ (phoQ) Protein, Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC), in vitro E.coli expression system, N-terminal 10xHis-tagged, 56.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Escherichia coli O6:H1 Sensor protein PhoQ (phoQ) Protein

Recombinant Escherichia coli O6:H1 Sensor protein PhoQ (phoQ) Protein — Member of the two-component regulatory system PhoP/PhoQ involved in virulence, adaptation to low Mg(2+) environments and the control of acid resistance genes. Purity >85%.

SKU: BCRECP-00132TM Category:
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Product Specifications

Uniprot No.Q8FIB8
Gene NamesphoQ
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-486aa
SpeciesEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Tag InfoN-terminal 10xHis-tagged
Molecular weight56.8kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Member of the two-component regulatory system PhoP/PhoQ involved in virulence, adaptation to low Mg(2+) environments and the control of acid resistance genes. Also involved in adaptation to hyperosmotic environments, in a Mg(2+)-independent manner. In low periplasmic Mg(2+), PhoQ functions as a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG).

Biological Context

Subcellular Location: Cell inner membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Escherichia coli O6:H1 Sensor protein PhoQ (phoQ) Protein is a recombinant protein from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC), expressed in in vitro E.coli expression system, covering amino acids 1-486aa, with N-terminal 10xHis-tagged tag, molecular weight 56.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Not Test

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Member of the two-component regulatory system PhoP/PhoQ involved in virulence, adaptation to low Mg(2+) environments and the control of acid resistance genes (By similarity). Also involved in adaptation to hyperosmotic environments, in a Mg(2+)-independent manner (By similarity). In low periplasmic Mg(2+), PhoQ functions as a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG) (By similarity). In high periplasmic Mg(2+), acts as a protein phosphatase that dephosphorylates phospho-PhoP, which results in the repression of PG and may lead to expression of some PRG (By similarity)

Subcellular Location

Cell inner membrane (Multi-pass membrane protein)

Subunit

Homodimer; probably dimerizes via the cytoplasmic domain. Probably interacts with MgrB in the periplasm, altering its activity and that of downstream effector PhoP. Interacts with two-component system connector protein SafA (via C-terminus)

Gene: phoQ  |  Organism: Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)  |  Synonyms: Sensor histidine protein kinase/phosphatase PhoQ

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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