Recombinant Escherichia coli O9:H4 Membrane protein insertase YidC (yidC) Protein, Escherichia coli O9:H4 (strain HS), in vitro E.coli expression system, N-terminal 10xHis-tagged, 63.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Escherichia coli O9:H4 Membrane protein insertase YidC (yidC) Protein

Recombinant Escherichia coli O9:H4 Membrane protein insertase YidC (yidC) Protein — Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Purity >85%.

SKU: BCRECP-00145TM Category:
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Product Specifications

Uniprot No.A8A6G7
Gene NamesyidC
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-548aa
SpeciesEscherichia coli O9:H4 (strain HS)
Tag InfoN-terminal 10xHis-tagged
Molecular weight63.0kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins.

Biological Context

Subcellular Location: Cell inner membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Escherichia coli O9:H4 Membrane protein insertase YidC (yidC) Protein is a recombinant protein from Escherichia coli O9:H4 (strain HS), expressed in in vitro E.coli expression system, covering amino acids 1-548aa, with N-terminal 10xHis-tagged tag, molecular weight 63.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins

Subcellular Location

Cell inner membrane (Multi-pass membrane protein)

Subunit

Interacts with the Sec translocase complex via SecD. Specifically interacts with transmembrane segments of nascent integral membrane proteins during membrane integration

Gene: yidC  |  Organism: Escherichia coli O9:H4 (strain HS)  |  Synonyms: Foldase YidC; Membrane integrase YidC; Membrane protein YidC

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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