Recombinant Escherichia coli Outer membrane protein assembly factor BamA (BamA), partial, Escherichia coli (strain K12), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 36.0kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli Outer membrane protein assembly factor BamA (BamA), partial

Recombinant Escherichia coli Outer membrane protein assembly factor BamA (BamA), partial — (Microbial infection) Acts as a receptor for CdiA-EC93, the contact-dependent growth inhibition (CDI) effector of E. Purity >90%.

SKU: BCRECP-002177 Category:
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Product Specifications

Product SkuBCRECP-002177
Product DescriptionRecombinant Escherichia coli Outer membrane protein assembly factor BamA (BamA) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 175-424aa. [Accession | P0A940].
Uniprot No.P0A940
Gene NamesbamA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region175-424aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight36.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

(Microbial infection) Acts as a receptor for CdiA-EC93, the contact-dependent growth inhibition (CDI) effector of E.coli strain EC93; antibodies against extracellular epitopes decrease CDI. Its role in CDI is independent of the other Bam complex components. Is not the receptor for CdiA from E.coli strain 536 / UPEC, which does not have the same mode of toxicity as CdiA from strain EC93; the decreased expression of bamA101 in some experiments decreases the level of outer membrane proteins in general.

Biological Context

Subcellular Location: Cell outer membrane

Product Specifications

Recombinant Escherichia coli Outer membrane protein assembly factor BamA (BamA), partial is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 175-424aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 36.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

(Microbial infection) Acts as a receptor for CdiA-EC93, the contact-dependent growth inhibition (CDI) effector of E.coli strain EC93; antibodies against extracellular epitopes decrease CDI. Its role in CDI is independent of the other Bam complex components (PubMed:18761695). Is not the receptor for CdiA from E.coli strain 536 / UPEC, which does not have the same mode of toxicity as CdiA from strain EC93; the decreased expression of bamA101 in some experiments decreases the level of outer membrane proteins in general (PubMed:23469034, PubMed:23882017). Susceptibility to CdiA-EC93 is dependent on E.coli BamA; replacing BamA with the gene from S.typhimurium LT2, E.cloacae ATCC 13047 or D.dadantii 3937 renders cells resistant to CdiA-EC93. Cells with BamA from another bacteria no longer form CdiA-EC93-induced aggregates with EC93 cells. A chimera in which E.cloacae extracellular loops 6 and 7 are replaced with loops 6 and 7 from E.coli is susceptible to CdiA-EC93 and to CdiA-CT from strain 536 / UPEC (PubMed:23882017)

Subcellular Location

Cell outer membrane

Subunit

Part of the Bam complex, which is composed of the outer membrane protein BamA, and four lipoproteins BamB, BamC, BamD and BamE. BamA interacts directly with BamB and the BamCDE subcomplex. The Bam complex has the shape of a hat, with the BamA beta-barrel crown in the outer membrane and the periplasmic brim formed by the BamA POTRA domains and the 4 lipoproteins (PubMed:26744406, PubMed:26900875, PubMed:26901871, PubMed:27686148)

Gene: bamA  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Omp85
Key Publications

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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