Recombinant Escherichia coli Peptide chain release factor 2 (prfB), Escherichia coli (strain K12), E.coli, C-terminal 6xHis-tagged, 48.2kDa, purity >95% as determined by SDS-PAGE.

Recombinant Escherichia coli Peptide chain release factor 2 (prfB)

Recombinant Escherichia coli Peptide chain release factor 2 (prfB) — Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA. Purity >95%.

SKU: BCRECP-004159 Category:
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Product Specifications

Product SkuBCRECP-004159
Product DescriptionRecombinant Escherichia coli Peptide chain release factor 2 (prfB) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-365aa. [Accession | P07012].
Uniprot No.P07012
Gene NamesprfB
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-365aa
SpeciesEscherichia coli (strain K12)
Tag InfoC-terminal 6xHis-tagged
Molecular weight48.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA. Acts as a peptidyl-tRNA hydrolase. In the presence of truncated mRNA in the 70S ribosome, ArfA and RF2 interact such that the GGQ peptide hydrolysis motif of RF2 rises into the peptidyl-transferase center (PTC) and releases the ribosome.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Escherichia coli Peptide chain release factor 2 (prfB) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 1-365aa, with C-terminal 6xHis-tagged tag, molecular weight 48.2kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA (PubMed:11118225, PubMed:17932046). Acts as a peptidyl-tRNA hydrolase (PubMed:22857598, PubMed:27934701). In the presence of truncated mRNA in the 70S ribosome, ArfA and RF2 interact such that the GGQ peptide hydrolysis motif of RF2 rises into the peptidyl-transferase center (PTC) and releases the ribosome (PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875). Recruited by ArfA to rescue stalled ribosomes in the absence of a normal stop codon (PubMed:22857598, PubMed:22922063, PubMed:25355516). A TnaC-stalled ribosome binds RF2, but the active GGQ motif is prevented from engaging with the PTC by TnaC (PubMed:34504068)

Subcellular Location

Cytoplasm

Subunit

Interacts with the ribosome (PubMed:12511960, PubMed:12511961, PubMed:22857598, PubMed:22922063, PubMed:25355516). Recruited to stalled ribosomes by ArfA, in the presence of truncated mRNA, ArfA influences RF2 conformation so RF2 can hydrolyze the peptidyl-tRNA bond (PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875). Biotinylated tnaC mRNA can be detected in a stalled 70S ribosome-RF2-TnaC-tRNA(Pro) complex (PubMed:34504068)

Gene: prfB  |  Organism: Escherichia coli (strain K12)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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