Recombinant Escherichia coli Peptidoglycan-associated lipoprotein (pal), Escherichia coli (strain K12), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 24.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Escherichia coli Peptidoglycan-associated lipoprotein (pal)

Recombinant Escherichia coli Peptidoglycan-associated lipoprotein (pal) — Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity. Purity >85%.

SKU: BCRECP-004019 Category:
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Product Specifications

Product SkuBCRECP-004019
Product DescriptionRecombinant Escherichia coli Peptidoglycan-associated lipoprotein (pal) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 22-173aa. [Accession | P0A912].
Uniprot No.P0A912
Gene Namespal
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region22-173aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight24.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity. The Tol-Pal system is also required for polar localization of chemoreceptors clusters. The system also appears to be required for the activity of several outer membrane-localized enzymes with cell wall remodeling activity.

Biological Context

Subcellular Location: Cell outer membrane (Lipid-anchor)

Product Specifications

Recombinant Escherichia coli Peptidoglycan-associated lipoprotein (pal) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 22-173aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 24.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity (PubMed:11115123, PubMed:17233825). The Tol-Pal system is also required for polar localization of chemoreceptors clusters (PubMed:24720726). The system also appears to be required for the activity of several outer membrane-localized enzymes with cell wall remodeling activity (PubMed:32152098)

Subcellular Location

Cell outer membrane (Lipid-anchor)

Subunit

The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer (PubMed:17233825). Pal interacts with TolB (PubMed:17375930, PubMed:19696740, PubMed:7744736). Interacts with the C-terminal domain of TolA. This interaction is proton motive force dependent and requires TolQ and TolR (PubMed:11115123, PubMed:11722743). Can form a complex with TolB and OmpA (PubMed:9701827)

Gene: pal  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Tol-Pal system lipoprotein Pal
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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