Recombinant Escherichia coli Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (pgaB), Escherichia coli (strain K12), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 82.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Escherichia coli Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (pgaB)

Recombinant Escherichia coli Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (pgaB) — Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide. Purity >85%.

SKU: BCRECP-004063 Category:
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Product Specifications

Product SkuBCRECP-004063
Product DescriptionRecombinant Escherichia coli Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (pgaB) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 21-672aa. [Accession | P75906].
Uniprot No.P75906
Gene NamespgaB
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region21-672aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight82.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide. N-deacetylation promotes PGA export through the PgaA porin.

Biological Context

Subcellular Location: Cell outer membrane (Lipid-anchor)

Product Specifications

Recombinant Escherichia coli Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (pgaB) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 21-672aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 82.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide. N-deacetylation promotes PGA export through the PgaA porin

Subcellular Location

Cell outer membrane (Lipid-anchor)

Gene: pgaB  |  Organism: Escherichia coli (strain K12)

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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