Recombinant Escherichia coli Polyphosphate kinase (ppk), Escherichia coli (strain K12), E.coli, N-terminal 6xHis-tagged, 84.4kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Escherichia coli Polyphosphate kinase (ppk)

Recombinant Escherichia coli Polyphosphate kinase (ppk) — Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). Purity >85%.

SKU: BCRECP-004144 Categories: , ,
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Product Specifications

Product SkuBCRECP-004144
Product DescriptionRecombinant Escherichia coli Polyphosphate kinase (ppk) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2-688aa. [Accession | P0A7B1].
Uniprot No.P0A7B1
Gene Namesppk
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-688aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-tagged
Molecular weight84.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). Can form linear polymers of orthophosphate with chain lengths up to 1000 or more. Can use GTP instead of ATP, but the efficiency of GTP is 5% that of ATP.

Biological Context

Subcellular Location: Cell membrane (Peripheral membrane protein)

Product Specifications

Recombinant Escherichia coli Polyphosphate kinase (ppk) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 2-688aa, with N-terminal 6xHis-tagged tag, molecular weight 84.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). Can form linear polymers of orthophosphate with chain lengths up to 1000 or more. Can use GTP instead of ATP, but the efficiency of GTP is 5% that of ATP. Also exhibits several other enzymatic activities, which include: ATP synthesis from polyP in the presence of excess ADP, general nucleoside-diphosphate kinase activity, linear guanosine 5'-tetraphosphate (ppppG) synthesis and autophosphorylation

Subcellular Location

Cell membrane (Peripheral membrane protein)

Subunit

Homotetramer (PubMed:1331061). Dimer of dimers (PubMed:10660553, PubMed:15947782). The diverse functions of this enzyme involve different subunit organizations and conformations (PubMed:10660553)

Gene: ppk  |  Organism: Escherichia coli (strain K12)  |  Synonyms: ATP-polyphosphate phosphotransferase; Polyphosphoric acid kinase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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