Recombinant Escherichia coli Polyphosphate kinase (ppk), Escherichia coli (strain K12), E.coli, N-terminal 6xHis-tagged, 84.4kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Escherichia coli Polyphosphate kinase (ppk)

Recombinant Escherichia coli Polyphosphate kinase (ppk) — Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). Purity >85%.

SKU: BCRECP-004144 Categories: , ,
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Product Specifications

Product SkuBCRECP-004144
Product DescriptionRecombinant Escherichia coli Polyphosphate kinase (ppk) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2-688aa. [Accession | P0A7B1].
Uniprot No.P0A7B1
Gene Namesppk
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-688aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-tagged
Molecular weight84.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). Can form linear polymers of orthophosphate with chain lengths up to 1000 or more. Can use GTP instead of ATP, but the efficiency of GTP is 5% that of ATP.

Biological Context

Subcellular Location: Cell membrane (Peripheral membrane protein)

Product Specifications

Recombinant Escherichia coli Polyphosphate kinase (ppk) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 2-688aa, with N-terminal 6xHis-tagged tag, molecular weight 84.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). Can form linear polymers of orthophosphate with chain lengths up to 1000 or more. Can use GTP instead of ATP, but the efficiency of GTP is 5% that of ATP. Also exhibits several other enzymatic activities, which include: ATP synthesis from polyP in the presence of excess ADP, general nucleoside-diphosphate kinase activity, linear guanosine 5'-tetraphosphate (ppppG) synthesis and autophosphorylation

Subcellular Location

Cell membrane (Peripheral membrane protein)

Subunit

Homotetramer (PubMed:1331061). Dimer of dimers (PubMed:10660553, PubMed:15947782). The diverse functions of this enzyme involve different subunit organizations and conformations (PubMed:10660553)

Gene: ppk  |  Organism: Escherichia coli (strain K12)  |  Synonyms: ATP-polyphosphate phosphotransferase; Polyphosphoric acid kinase
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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