Recombinant Escherichia coli Putative binding protein ygiS (ygiS), Escherichia coli (strain K12), E.coli, N-terminal 6xHis-SUMO-tagged, 74.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli Putative binding protein ygiS (ygiS)

Recombinant Escherichia coli Putative binding protein ygiS (ygiS) — Probably part of a deoxycholate transport system. Purity >90%.

SKU: BCRECP-004767 Category:
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Product Specifications

Product SkuBCRECP-004767
Product DescriptionRecombinant Escherichia coli Putative binding protein ygiS (ygiS) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 21-535aa. [Accession | Q46863].
Uniprot No.Q46863
Gene NamesygiS
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region21-535aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight74.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Probably part of a deoxycholate transport system. Its expression in the presence of deoxycholate in a ygiS deletion mutant increases intracellular deoxycholate levels and decreases cell growth; higher expression in the presence of deoxycholate inhibits cell growth completely. Bile acid detergents such as deoxycholate are important for host defense against bacterial growth in the gall bladder and duodenum.

Biological Context

Subcellular Location: Periplasm

Product Specifications

Recombinant Escherichia coli Putative binding protein ygiS (ygiS) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 21-535aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 74.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Probably part of a deoxycholate transport system. Its expression in the presence of deoxycholate in a ygiS deletion mutant increases intracellular deoxycholate levels and decreases cell growth; higher expression in the presence of deoxycholate inhibits cell growth completely. Bile acid detergents such as deoxycholate are important for host defense against bacterial growth in the gall bladder and duodenum

Subcellular Location

Periplasm

Gene: ygiS  |  Organism: Escherichia coli (strain K12)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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