Recombinant Escherichia coli Pyruvate kinase I (pykF), Escherichia coli (strain K12), E.coli, N-terminal 6xHis-tagged, 54.8kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Escherichia coli Pyruvate kinase I (pykF)

Recombinant Escherichia coli Pyruvate kinase I (pykF) — Catalyzes the formation of pyruvate in the last step of glycolysis, it is irreversible under physiological conditions. Purity >90%.

SKU: BCRECP-004221 Categories: , ,
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Product Specifications

Product SkuBCRECP-004221
Product DescriptionRecombinant Escherichia coli Pyruvate kinase I (pykF) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-470aa. [Accession | P0AD61].
Uniprot No.P0AD61
Gene NamespykF
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-470aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-tagged
Molecular weight54.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Catalyzes the formation of pyruvate in the last step of glycolysis, it is irreversible under physiological conditions. The reaction is critical for the control of metabolic flux in the second part of glycolysis.

Biological Context

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5

Product Specifications

Recombinant Escherichia coli Pyruvate kinase I (pykF) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 1-470aa, with N-terminal 6xHis-tagged tag, molecular weight 54.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Catalyzes the formation of pyruvate in the last step of glycolysis, it is irreversible under physiological conditions. The reaction is critical for the control of metabolic flux in the second part of glycolysis

Subunit

Homotetramer

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5

Gene: pykF  |  Organism: Escherichia coli (strain K12)  |  Synonyms: PK-1
Key Publications

Explore closely related product collections, technology platforms, and custom production resources.

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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