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Recombinant Escherichia coli Relaxosome protein TraY (traY)

Recombinant Escherichia coli Relaxosome protein TraY (traY) — Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. Purity >85%.

SKU: BCRECP-004613 Category:
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Product Specifications

Product SkuBCRECP-004613
Product DescriptionRecombinant Escherichia coli Relaxosome protein TraY (traY) Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 1-75aa. [Accession | P10512].
Uniprot No.P10512
Gene NamestraY
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-75aa
SpeciesEscherichia coli
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight24.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Escherichia coli Relaxosome protein TraY (traY) is a recombinant protein from Escherichia coli, expressed in E.coli, covering amino acids 1-75aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 24.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Relaxosome formation requires binding of IHF and TraY to the oriT region, which then facilitates binding of TraI. Also positively regulates tra gene expression (By similarity)

Subcellular Location

Cytoplasm

Subunit

Part of the relaxosome, a complex composed of plasmid encoded TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of transfer (oriT). Interacts with TraM, probably through its C-terminus (By similarity)

Gene: traY  |  Organism: Escherichia coli

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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