Recombinant Escherichia coli Respiratory nitrate reductase 1 alpha chain (narG), partial, Escherichia coli (strain K12), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 20.5kDa, purity >90% as determined by SDS-PAGE.
,

Recombinant Escherichia coli Respiratory nitrate reductase 1 alpha chain (narG), partial

Recombinant Escherichia coli Respiratory nitrate reductase 1 alpha chain (narG), partial — The nitrate reductase enzyme complex allows E. Purity >90%.

SKU: BCRECP-003849 Categories: ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-003849
Product DescriptionRecombinant Escherichia coli Respiratory nitrate reductase 1 alpha chain (narG) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-110aa. [Accession | P09152].
Uniprot No.P09152
Gene NamesnarG
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-110aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight20.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The alpha chain is the actual site of nitrate reduction.

Biological Context

Subcellular Location: Cell membrane (Peripheral membrane protein)

Product Specifications

Recombinant Escherichia coli Respiratory nitrate reductase 1 alpha chain (narG), partial is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 1-110aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 20.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The alpha chain is the actual site of nitrate reduction

Subcellular Location

Cell membrane (Peripheral membrane protein)

Subunit

Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane. Interacts with the NarJ chaperone

Gene: narG  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Nitrate reductase A subunit alpha; Quinol-nitrate oxidoreductase subunit alpha
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top