Recombinant Escherichia coli Ribonuclease E (rne), partial, Escherichia coli (strain K12), E.coli, N-terminal 6xHis-tagged, 16.1kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli Ribonuclease E (rne), partial

Recombinant Escherichia coli Ribonuclease E (rne), partial — Prefers 5′-monophosphorylated substrates over 5′-triphosphorylated substrates. Purity >90%.

SKU: BCRECP-004278 Category:
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Product Specifications

Product SkuBCRECP-004278
Product DescriptionRecombinant Escherichia coli Ribonuclease E (rne) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 35-125aa. [Accession | P21513].
Uniprot No.P21513
Gene Namesrne
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region35-125aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-tagged
Molecular weight16.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Prefers 5'-monophosphorylated substrates over 5'-triphosphorylated substrates. 5'-monophosphate-assisted cleavage requires at least 2 and preferably 3 or more unpaired 5'-terminal nucleotides. The optimal spacing between the 5' end and the scissile phosphate appears to be 8 nucleotides.

Biological Context

Subcellular Location: Cytoplasm; Cell inner membrane (Peripheral membrane protein)

Product Specifications

Recombinant Escherichia coli Ribonuclease E (rne), partial is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 35-125aa, with N-terminal 6xHis-tagged tag, molecular weight 16.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Prefers 5'-monophosphorylated substrates over 5'-triphosphorylated substrates (PubMed:10762247). 5'-monophosphate-assisted cleavage requires at least 2 and preferably 3 or more unpaired 5'-terminal nucleotides. The optimal spacing between the 5' end and the scissile phosphate appears to be 8 nucleotides. Any sequence of unpaired nucleotides at the 5'-end is tolerated (PubMed:26694614)

Subcellular Location

Cytoplasm; Cell inner membrane (Peripheral membrane protein)

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers. Tetramerization is essential for catalytic activity, but not for RNA-binding. Interacts with RhlB, PNPase (pnp) and enolase (eno). Interacts with DeaD at reduced temperature

Gene: rne  |  Organism: Escherichia coli (strain K12)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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