Recombinant Escherichia coli Ribonuclease E (rne), partial, Escherichia coli (strain K12), E.coli, N-terminal 6xHis-tagged, 16.1kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli Ribonuclease E (rne), partial

Recombinant Escherichia coli Ribonuclease E (rne), partial — Prefers 5′-monophosphorylated substrates over 5′-triphosphorylated substrates. Purity >90%.

SKU: BCRECP-004278 Category:
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Product Specifications

Product SkuBCRECP-004278
Product DescriptionRecombinant Escherichia coli Ribonuclease E (rne) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 35-125aa. [Accession | P21513].
Uniprot No.P21513
Gene Namesrne
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region35-125aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-tagged
Molecular weight16.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Prefers 5'-monophosphorylated substrates over 5'-triphosphorylated substrates. 5'-monophosphate-assisted cleavage requires at least 2 and preferably 3 or more unpaired 5'-terminal nucleotides. The optimal spacing between the 5' end and the scissile phosphate appears to be 8 nucleotides.

Biological Context

Subcellular Location: Cytoplasm; Cell inner membrane (Peripheral membrane protein)

Product Specifications

Recombinant Escherichia coli Ribonuclease E (rne), partial is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 35-125aa, with N-terminal 6xHis-tagged tag, molecular weight 16.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Prefers 5'-monophosphorylated substrates over 5'-triphosphorylated substrates (PubMed:10762247). 5'-monophosphate-assisted cleavage requires at least 2 and preferably 3 or more unpaired 5'-terminal nucleotides. The optimal spacing between the 5' end and the scissile phosphate appears to be 8 nucleotides. Any sequence of unpaired nucleotides at the 5'-end is tolerated (PubMed:26694614)

Subcellular Location

Cytoplasm; Cell inner membrane (Peripheral membrane protein)

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers. Tetramerization is essential for catalytic activity, but not for RNA-binding. Interacts with RhlB, PNPase (pnp) and enolase (eno). Interacts with DeaD at reduced temperature

Gene: rne  |  Organism: Escherichia coli (strain K12)
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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