Recombinant Escherichia coli Transketolase 1 (tktA), Escherichia coli (strain K12), E.coli, N-terminal 6xHis-SUMO-tagged, 88.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli Transketolase 1 (tktA)

Recombinant Escherichia coli Transketolase 1 (tktA) — Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate. Purity >90%.

SKU: BCRECP-004571 Category:
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Product Specifications

Product SkuBCRECP-004571
Product DescriptionRecombinant Escherichia coli Transketolase 1 (tktA) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-663aa. [Accession | P27302].
Uniprot No.P27302
Gene NamestktA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-663aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight88.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate. Thus, catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate.

Biological Context

Pathway: Carbohydrate degradation; pentose phosphate pathway

Product Specifications

Recombinant Escherichia coli Transketolase 1 (tktA) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 1-663aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 88.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate. Thus, catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate

Subunit

Homodimer

Pathway

Carbohydrate degradation; pentose phosphate pathway

Gene: tktA  |  Organism: Escherichia coli (strain K12)
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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