Recombinant Escherichia coli Uncharacterized protein YcbX (ycbX) (Active), Escherichia (strain K12), E.coli, N-terminal GST-tagged, 67.6 kDa, purity >85% as determined by SDS-PAGE.

Recombinant Escherichia coli Uncharacterized protein YcbX (ycbX) (Active)

Recombinant Escherichia coli Uncharacterized protein YcbX (ycbX) (Active) — Reductase involved in the detoxification of N-hydroxylated base analogs. Purity >85%.

SKU: BCACP-000042 Category:
Online Inquiry →
Product SKUBCACP-000042
Product DescriptionRecombinant strain K12 coli Uncharacterized protein YcbX (ycbX) protein is expressed from E.coli with N-terminal GST-tagged. It contains 1-369aa. [Accession | P75863].
Uniprot No.P75863
Gene NamesycbX
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-369aa
SpeciesEscherichia (strain K12)
Tag InfoN-terminal GST-tagged
Molecular weight67.6 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized ldcC at 5 μg/ml can bind Escherichia coli ycbX, the EC50 of Escherichia coli ycbX protein is 359.062 - 628.116 μg/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesycbX; b0947; JW5126; Uncharacterized protein YcbX

Function

Reductase involved in the detoxification of N-hydroxylated base analogs. Contributes to the detoxification of 6-N-hydroxylaminopurine (HAP) by catalyzing the reduction of HAP to adenine. In the presence of methyl viologen as reduced electron donor, it is also active toward other N-hydroxy substrates such as 2-amino-6-hydroxylaminopurine (2-AHAP), N-hydroxyadenosine, N-hydroxyacetamidine, N-hydroxyurea and hydroxylamine, but it cannot use N-oxide and sulfoxide substrates, or nitrate and chlorate.

Product Specifications

Recombinant Escherichia coli Uncharacterized protein YcbX (ycbX) (Active) is a recombinant protein from Escherichia (strain K12), expressed in E.coli, covering amino acids 1-369aa, with N-terminal GST-tagged tag, molecular weight 67.6 kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized ldcC at 5 μg/ml can bind Escherichia coli ycbX, the EC50 of Escherichia coli ycbX protein is 359.062 - 628.116 μg/ml.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Reductase involved in the detoxification of N-hydroxylated base analogs (PubMed:18312271). Contributes to the detoxification of 6-N-hydroxylaminopurine (HAP) by catalyzing the reduction of HAP to adenine (PubMed:18312271, PubMed:35640667). In the presence of methyl viologen as reduced electron donor, it is also active toward other N-hydroxy substrates such as 2-amino-6-hydroxylaminopurine (2-AHAP), N-hydroxyadenosine, N-hydroxyacetamidine, N-hydroxyurea and hydroxylamine, but it cannot use N-oxide and sulfoxide substrates, or nitrate and chlorate (PubMed:35640667). The reducing equivalents required for the detoxification reaction may be provided by the sulfite reductase component CysJ, which functions as a partner of YcbX (PubMed:20118259). YcbX is not involved in molybdenum cofactor (MoCo) biosynthesis or in MoCo sulfuration (PubMed:18312271)

Gene: ycbX  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Hydroxylaminopurine resistance molybdoenzyme YcbX
Key Publications

Explore closely related product collections, technology platforms, and custom production resources.

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

Shopping Cart
Scroll to Top