Recombinant Escherichia phage T7 T7 RNA polymerase (1), partial, Escherichia phage T7 (Bacteriophage T7), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 34.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia phage T7 T7 RNA polymerase (1), partial

Recombinant Escherichia phage T7 T7 RNA polymerase (1), partial — Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Purity >90%.

SKU: BCRECP-001954 Category:
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Product Specifications

Product SkuBCRECP-001954
Product DescriptionRecombinant Escherichia phage T7 T7 RNA polymerase (1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 274-509aa. [Accession | P00573].
Uniprot No.P00573
Gene Names1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region274-509aa
SpeciesEscherichia phage T7 (Bacteriophage T7)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight34.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Recognizes a specific promoter sequence and enters first into an 'abortive phase' where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. Unwinds the double-stranded DNA to expose the coding strand for templating.

Product Specifications

Recombinant Escherichia phage T7 T7 RNA polymerase (1), partial is a recombinant protein from Escherichia phage T7 (Bacteriophage T7), expressed in E.coli, covering amino acids 274-509aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 34.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Recognizes a specific promoter sequence and enters first into an 'abortive phase' where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. Unwinds the double-stranded DNA to expose the coding strand for templating. Participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Also plays a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process

Subunit

Monomer. Interacts with T7 lysozyme; this interaction inhibits transcriptional function of T7 RNA polymerase

Organism: Escherichia phage T7  |  Synonyms: DNA-directed RNA polymerase
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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