Recombinant Francisella tularensis subsp. novicida Succinyl-CoA ligase [ADP-forming] subunit beta (sucC), Francisella tularensis subsp. novicida (strain U112), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 49.0kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Francisella tularensis subsp. novicida Succinyl-CoA ligase [ADP-forming] subunit beta (sucC)

Recombinant Francisella tularensis subsp. novicida Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) — Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate…

SKU: BCRECP-004511 Categories: ,
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Product Specifications

Product SkuBCRECP-004511
Product DescriptionRecombinant Francisella tularensis subsp. novicida Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-387aa. [Accession | A0Q5H4].
Uniprot No.A0Q5H4
Gene NamessucC
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-387aa
SpeciesFrancisella tularensis subsp. novicida (strain U112)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight49.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.

Biological Context

Pathway: Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1

Product Specifications

Recombinant Francisella tularensis subsp. novicida Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) is a recombinant protein from Francisella tularensis subsp. novicida (strain U112), expressed in E.coli, covering amino acids 1-387aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 49.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit

Subunit

Heterotetramer of two alpha and two beta subunits

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1

Gene: sucC  |  Organism: Francisella tularensis subsp. novicida (strain ATCC 15482 / CCUG 33449 / U112)  |  Synonyms: Succinyl-CoA synthetase subunit beta

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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