Recombinant Glycine max Seed biotin-containing protein SBP65 (SBP65), partial

Recombinant Glycine max Seed biotin-containing protein SBP65 (SBP65), partial — recombinant protein from Glycine max (Soybean) (Glycine hispida), expressed in E.coli. Purity >90%.

SKU: BCRECP-004376 Category:

Product Specifications

Product SkuBCRECP-004376
Product DescriptionRecombinant Glycine max Seed biotin-containing protein SBP65 (SBP65) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-255aa. [Accession | Q39846].
Uniprot No.Q39846
Gene NamesSBP65
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-255aa
SpeciesGlycine max (Soybean) (Glycine hispida)
Tag InfoC-terminal 6xHis-tagged
Molecular weight34.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

May serve as a biotin provider for several growth-limiting enzymes that are necessary during seed development and the subsequent germination stages, and thus may play some roles in determining seed germination capacity.

Biological Context

Tissue Specificity: Expressed in the leaf primodium and the vascular tissues of the hypocotyl-radicle axis of mature seeds. High protein levels in dry and mature soybean seeds, but not found in fresh immature seeds

Product Specifications

Recombinant Glycine max Seed biotin-containing protein SBP65 (SBP65), partial is a recombinant protein from Glycine max (Soybean) (Glycine hispida), expressed in E.coli, covering amino acids 1-255aa, with C-terminal 6xHis-tagged tag, molecular weight 34.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Protein Biology

Function

May serve as a biotin provider for several growth-limiting enzymes that are necessary during seed development and the subsequent germination stages, and thus may play some roles in determining seed germination capacity

Tissue Specificity

Expressed in the leaf primodium and the vascular tissues of the hypocotyl-radicle axis of mature seeds. High protein levels in dry and mature soybean seeds, but not found in fresh immature seeds

Gene: SBP65  |  Organism: Glycine max  |  Synonyms: BP75; Seed biotinylated protein of 65 kDa

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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