Recombinant Hepatitis B virus genotype C subtype ayw Capsid protein (C), Hepatitis B virus genotype C subtype ayw (isolate China/Tibet127/2002) (HBV-C), E.coli, N-terminal 10xHis-tagged, 27.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Hepatitis B virus genotype C subtype ayw Capsid protein (C)

Recombinant Hepatitis B virus genotype C subtype ayw Capsid protein (C) — Self assembles to form an icosahedral capsid. Purity >90%.

SKU: BCRECP-002249 Categories: , ,
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Product Specifications

Product SkuBCRECP-002249
Product DescriptionRecombinant Hepatitis B virus genotype C subtype ayw Capsid protein (C) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 1-183aa. [Accession | P0C6H7].
Uniprot No.P0C6H7
Gene NamesC
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-183aa
SpeciesHepatitis B virus genotype C subtype ayw (isolate China/Tibet127/2002) (HBV-C)
Tag InfoN-terminal 10xHis-tagged
Molecular weight27.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Self assembles to form an icosahedral capsid. Most capsids appear to be large particles with an icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsids are transported along microtubules to the nucleus.

Biological Context

Subcellular Location: Virion; Host cytoplasm

Product Specifications

Recombinant Hepatitis B virus genotype C subtype ayw Capsid protein (C) is a recombinant protein from Hepatitis B virus genotype C subtype ayw (isolate China/Tibet127/2002) (HBV-C), expressed in E.coli, covering amino acids 1-183aa, with N-terminal 10xHis-tagged tag, molecular weight 27.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Self assembles to form an icosahedral capsid. Most capsids appear to be large particles with an icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsids are transported along microtubules to the nucleus. Phosphorylation of the capsid is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex via the importin (karyopherin-) alpha and beta. Capsids are imported in intact form through the nuclear pore into the nuclear basket, where it probably binds NUP153. Only capsids that contain the mature viral genome can release the viral DNA and capsid protein into the nucleoplasm. Immature capsids get stuck in the basket. Capsids encapsulate the pre-genomic RNA and the P protein. Pre-genomic RNA is reverse-transcribed into DNA while the capsid is still in the cytoplasm. The capsid can then either be directed to the nucleus, providing more genomes for transcription, or bud through the endoplasmic reticulum to provide new virions

Subcellular Location

Virion; Host cytoplasm

Subunit

Homodimerizes, then multimerizes. Interacts with cytosol exposed regions of viral L glycoprotein present in the reticulum-to-Golgi compartment. Interacts with human FLNB. Phosphorylated form interacts with host importin alpha; this interaction depends on the exposure of the NLS, which itself depends upon genome maturation and/or phosphorylation of the capsid protein. Interacts with host NUP153

Gene: C  |  Organism: Hepatitis B virus genotype C subtype ayw (isolate China/Tibet127/2002)  |  Synonyms: Core antigen; Core protein; HBcAg; p21.5

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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