Recombinant Hepatitis E virus genotype 1 Protein ORF3 (ORF3), Hepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1), E.coli, N-terminal 6xHis-SUMO-tagged, 27.8kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Hepatitis E virus genotype 1 Protein ORF3 (ORF3)

Recombinant Hepatitis E virus genotype 1 Protein ORF3 (ORF3) — Small multifunctional phosphoprotein involved in virion morphogenesis, egress and counteracting host innate immunity. Purity >90%.

SKU: BCRECP-003971 Categories: , ,
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Product Specifications

Product SkuBCRECP-003971
Product DescriptionRecombinant Hepatitis E virus genotype 1 Protein ORF3 (ORF3) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-114aa. [Accession | O90299].
Uniprot No.O90299
Gene NamesORF3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-114aa
SpeciesHepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight27.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Small multifunctional phosphoprotein involved in virion morphogenesis, egress and counteracting host innate immunity. Plays critical roles in the final steps of viral release by interacting with host TSG101, a member of the vacuolar protein-sorting pathway and using other cellular host proteins involved in vesicle formation pathway. Also acts as a viroporin and forms ion conductive pores allowing viral particle release.

Biological Context

Subcellular Location: Host endoplasmic reticulum membrane (Lipid-anchor); Host cytoplasm, host cytoskeleton; Virion; Host cell membrane (Lipid-anchor)

Product Specifications

Recombinant Hepatitis E virus genotype 1 Protein ORF3 (ORF3) is a recombinant protein from Hepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1), expressed in E.coli, covering amino acids 1-114aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 27.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Small multifunctional phosphoprotein involved in virion morphogenesis, egress and counteracting host innate immunity. Plays critical roles in the final steps of viral release by interacting with host TSG101, a member of the vacuolar protein-sorting pathway and using other cellular host proteins involved in vesicle formation pathway. Also acts as a viroporin and forms ion conductive pores allowing viral particle release. Impairs the generation of type I interferon by down-regulating host TLR3 and TLR7 as well as their downstream signaling pathways. Down-regulates the phosphorylation of host IRF3 via the interaction with host SIRP-alpha, thereby inhibiting IFN-I expression. Interacts with host microtubules

Subcellular Location

Host endoplasmic reticulum membrane (Lipid-anchor); Host cytoplasm, host cytoskeleton; Virion; Host cell membrane (Lipid-anchor)

Subunit

Forms homooligomers (By similarity). Interacts with host SRC, HCK, FYN, PIK3R3 and GRB2 (via SH3 domain); binding does not activate the kinases. Interacts with host AMBP/bikunin and AMBP/alpha-1-microglobulin peptides. Interacts with host HPX/hemopexin. Interacts (when phosphorylated) with capsid protein ORF2 (PubMed:11160756, PubMed:11518702, PubMed:11934888, PubMed:15037615, PubMed:16140784, PubMed:18211098). Interacts with host TSG101; this interaction plays a role in viral release from the host cell (By similarity). Interacts with host SIRPA; this interaction down-regulates the phosphorylation of host IRF3 (By similarity)

Organism: Hepatitis E virus genotype 1 (isolate Human/India/Hyderabad)
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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