Recombinant Hepatitis E virus genotype 3 Pro-secreted protein ORF2 (ORF2), partial, Hepatitis E virus genotype 3 (isolate Human/United States/US2) (HEV-3), E.coli, N-terminal 6xHis-tagged, 31.4kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Hepatitis E virus genotype 3 Pro-secreted protein ORF2 (ORF2), partial

Recombinant Hepatitis E virus genotype 3 Pro-secreted protein ORF2 (ORF2), partial — Forms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. Purity >85%.

SKU: BCRECP-003969 Categories: , ,
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Product Specifications

Product SkuBCRECP-003969
Product DescriptionRecombinant Hepatitis E virus genotype 3 Pro-secreted protein ORF2 (ORF2) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 411-660aa. [Accession | Q9YLQ9].
Uniprot No.Q9YLQ9
Gene NamesORF2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region411-660aa
SpeciesHepatitis E virus genotype 3 (isolate Human/United States/US2) (HEV-3)
Tag InfoN-terminal 6xHis-tagged
Molecular weight31.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Forms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60 copies linked to each other. Binds to the 5' end of the genomic RNA to mediate genome encapsidation.

Biological Context

Subcellular Location: Virion; Host cytoplasm; Host endoplasmic reticulum; Host Golgi apparatus; Host cell surface; Host nucleus

Product Specifications

Recombinant Hepatitis E virus genotype 3 Pro-secreted protein ORF2 (ORF2), partial is a recombinant protein from Hepatitis E virus genotype 3 (isolate Human/United States/US2) (HEV-3), expressed in E.coli, covering amino acids 411-660aa, with N-terminal 6xHis-tagged tag, molecular weight 31.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Forms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60 copies linked to each other. Binds to the 5' end of the genomic RNA to mediate genome encapsidation (By similarity). Binds to heparin surface proteoglycans (HSPGs) to mediate viral entry. Additionally, the interactions with host ASGR1 and ASGR2 facilitate viral infection of hepatocytes (By similarity). Inhibits IFN production by blocking host TBK1-induced IRF3 phosphorylation (By similarity). The nuclear form probably modulates host gene expression (By similarity)

Subcellular Location

Virion; Host cytoplasm; Host endoplasmic reticulum; Host Golgi apparatus; Host cell surface; Host nucleus

Subunit

Self-assembles to form the capsid. The capsid is dominated by dimers that define the 30 morphological units. Interacts with phosphorylated protein ORF3 (By similarity). Interacts with host TMEM134. Interacts with host ASGR1 and ASGR2; these interactions facilitate infection of host hepatocytes (By similarity)

Organism: Hepatitis E virus genotype 3 (isolate Human/United States/US2)  |  Synonyms: Protein ORF2

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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