Recombinant Human 14-3-3 protein sigma (SFN), Homo sapiens (Human), E.coli, N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged, 62.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human 14-3-3 protein sigma (SFN)

Recombinant Human 14-3-3 protein sigma (SFN) — Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Purity >85%.

SKU: BCRECP-001624 Category:
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Product Specifications

Product SkuBCRECP-001624
Product DescriptionRecombinant Human 14-3-3 protein sigma (SFN) Protein is expressed from E.coli with N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged. It contains 1-248aa. [Accession | P31947].
Uniprot No.P31947
Gene NamesSFN
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-248aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-GST-tagged and C-terminal Myc-tagged
Molecular weight62.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Biological Context

Subcellular Location: Cytoplasm; Nucleus; Secreted
Tissue Specificity: Present mainly in tissues enriched in stratified squamous keratinizing epithelium

Product Specifications

Recombinant Human 14-3-3 protein sigma (SFN) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-248aa, with N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged tag, molecular weight 62.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binding generally results in the modulation of the activity of the binding partner (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed:37797010). Also acts as a TP53/p53-regulated inhibitor of G2/M progression (PubMed:9659898). When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis (PubMed:24124604). Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion (PubMed:29678907). May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (PubMed:18382127)

Subcellular Location

Cytoplasm; Nucleus; Secreted

Tissue Specificity

Present mainly in tissues enriched in stratified squamous keratinizing epithelium

Subunit

Homodimer (PubMed:28202711). Interacts with KRT17 and SAMSN1 (By similarity). Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29 and VPS35 (PubMed:15282546). Interacts with GAB2 (PubMed:19172738). Interacts with SRPK2 (PubMed:19592491). Interacts with COPS6 (PubMed:21625211). Interacts with COP1; this interaction leads to proteasomal degradation (PubMed:21625211). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB (PubMed:23572552). Interacts with SLITRK1 (PubMed:19640509). Interacts with LRRK2; this interaction is dependent on LRRK2 phosphorylation (PubMed:28202711). Interacts with PKP3 (via N-terminus); the interaction maintains the cytoplasmic pool of PKP3, facilitates PKP3 exchange at desmosomes and restricts PKP3 localization to existing desmosome cell junctions (PubMed:24124604, PubMed:29678907). Interacts with LCP2 (PubMed:33159816)

Gene: SFN  |  Organism: Homo sapiens  |  Synonyms: Epithelial cell marker protein 1; Stratifin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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