Recombinant Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-KSI-tagged, 20.0kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2), partial

Recombinant Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2), partial — Catalyzes the irreversible stereospecific reduction of the delta 4,5 bond (double bond between carbons 4 and 5) of various 3-oxo steroids (3-keto steroids) producing their 5alpha dihydro-3-oxo forms. Purity >85%.

SKU: BCRECP-001693 Categories: ,
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Product Specifications

Product SkuBCRECP-001693
Product DescriptionRecombinant Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 29-71aa. [Accession | P31213].
Uniprot No.P31213
Gene NamesSRD5A2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region29-71aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight20.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Catalyzes the irreversible stereospecific reduction of the delta 4,5 bond (double bond between carbons 4 and 5) of various 3-oxo steroids (3-keto steroids) producing their 5alpha dihydro-3-oxo forms. Converts testosterone into 5-alpha-dihydrotestosterone (DHT), the most active androgen in the prostate, as it is the preferred ligand for androgen receptor (AR) transactivation, making this reaction a key step in male sexual differentiation during development. Besides testosterone, it can also act on other steroids, including progesterone, producing metabolites with diverse roles.

Biological Context

Subcellular Location: Microsome membrane (Multi-pass membrane protein); Endoplasmic reticulum membrane (Multi-pass membrane protein)
Tissue Specificity: Expressed in high levels in the prostate and many other androgen-sensitive tissues
Disease Association: Pseudovaginal perineoscrotal hypospadias (PPSH) : A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 29-71aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 20.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Catalyzes the irreversible stereospecific reduction of the delta 4,5 bond (double bond between carbons 4 and 5) of various 3-oxo steroids (3-keto steroids) producing their 5alpha dihydro-3-oxo forms (PubMed:10898110, PubMed:23685396). Converts testosterone into 5-alpha-dihydrotestosterone (DHT), the most active androgen in the prostate, as it is the preferred ligand for androgen receptor (AR) transactivation, making this reaction a key step in male sexual differentiation during development (PubMed:10898110, PubMed:23685396). Besides testosterone, it can also act on other steroids, including progesterone, producing metabolites with diverse roles (PubMed:10898110). Hence, it plays a central role in sexual differentiation and androgen physiology (PubMed:10898110)

Subcellular Location

Microsome membrane (Multi-pass membrane protein); Endoplasmic reticulum membrane (Multi-pass membrane protein)

Disease Association

Pseudovaginal perineoscrotal hypospadias (PPSH) : A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in high levels in the prostate and many other androgen-sensitive tissues

Gene: SRD5A2  |  Organism: Homo sapiens  |  Synonyms: 5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; Type II 5-alpha reductase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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