Recombinant Human Actin, alpha cardiac muscle 1 (ACTC1), partial, Homo sapiens (Human), Baculovirus, C-terminal 6xHis-tagged, 47.4kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Actin, alpha cardiac muscle 1 (ACTC1), partial

Recombinant Human Actin, alpha cardiac muscle 1 (ACTC1), partial — recombinant protein from Homo sapiens (Human), expressed in Baculovirus. Purity >95%.

SKU: BCRECP-000027 Category:
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Product Specifications

Product SkuBCRECP-000027
Product DescriptionRecombinant Human Actin, alpha cardiac muscle 1 (ACTC1) Protein is expressed from Baculovirus with C-terminal 6xHis-tagged. It contains 3-377aa. [Accession | P68032].
Uniprot No.P68032
Gene NamesACTC1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region3-377aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight47.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Biological Context

Subcellular Location: Cytoplasm, cytoskeleton
Disease Association: Cardiomyopathy, dilated, 1R (CMD1R) : A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. [The disease is caused by variants affecting the gene represented in this entry] | Cardiomyopathy, familial hypertrophic, 11 (CMH11) : A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. [The disease is caused by variants affecting the gene represented in this entry] | Atrial septal defect 5 (ASD5) : A congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Actin, alpha cardiac muscle 1 (ACTC1), partial is a recombinant protein from Homo sapiens (Human), expressed in Baculovirus, covering amino acids 3-377aa, with C-terminal 6xHis-tagged tag, molecular weight 47.4kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells

Subcellular Location

Cytoplasm, cytoskeleton

Disease Association

Cardiomyopathy, dilated, 1R (CMD1R) : A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. [The disease is caused by variants affecting the gene represented in this entry] | Cardiomyopathy, familial hypertrophic, 11 (CMH11) : A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. [The disease is caused by variants affecting the gene represented in this entry] | Atrial septal defect 5 (ASD5) : A congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others

Gene: ACTC1  |  Organism: Homo sapiens  |  Synonyms: Alpha-cardiac actin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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