Recombinant Human Anaphase-promoting complex subunit 15 (ANAPC15), Homo sapiens (Human), E.coli, N-terminal GST-tagged, 41.3kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Anaphase-promoting complex subunit 15 (ANAPC15)

Recombinant Human Anaphase-promoting complex subunit 15 (ANAPC15) — Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through the cell cycle. Purity >90%.

SKU: BCRECP-000073 Category:
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Product Specifications

Product SkuBCRECP-000073
Product DescriptionRecombinant Human Anaphase-promoting complex subunit 15 (ANAPC15) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 1-121aa. [Accession | P60006].
Uniprot No.P60006
Gene NamesANAPC15
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-121aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight41.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through the cell cycle. APC/C acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. APC/C catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins.

Biological Context

Pathway: Protein modification; protein ubiquitination

Product Specifications

Recombinant Human Anaphase-promoting complex subunit 15 (ANAPC15) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-121aa, with N-terminal GST-tagged tag, molecular weight 41.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through the cell cycle (PubMed:21926987, PubMed:27120157, PubMed:27509861). APC/C acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:29033132). APC/C catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132). APC/C is activated by CDC20 in the metaphase/anaphase transition of cell cycle, targeting the degradation of cyclin B and securin (PubMed:27120157, PubMed:27509861). APC/C is regulated by the mitotic checkpoint complex (MCC), which inhibits APC/C and delays chromosome segregation (PubMed:21926987, PubMed:27509861). Also required for degradation of CDC20 (PubMed:21926987)

Subunit

Component of the anaphase promoting complex/cyclosome (APC/C), composed of ANAPC1, ANAPC2, CDC27/ANAPC3, ANAPC4, ANAPC5, CDC16/ANAPC6, ANAPC7, CDC23/ANAPC8, ANAPC10, ANAPC11, CDC26/ANAPC12, ANAPC13, ANAPC15 and ANAPC16 (PubMed:21926987, PubMed:25043029, PubMed:26083744, PubMed:27120157, PubMed:27509861). APC/C associates with CDC20 to form the CDC20-APC/C complex, which is crucial for metaphase/anaphase transition in cell cycle; this interaction is dependent on APC/C phosphorylation (PubMed:27120157, PubMed:27509861). APC/C associates with mitotic checkpoint complex (MCC), which inhibits APC/C and delays chromosome segregation (PubMed:27509861). APC/C interacts with FZR1 and FBXO5 (PubMed:25043029, PubMed:26083744)

Pathway

Protein modification; protein ubiquitination

Gene: ANAPC15  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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