Recombinant Human Apolipoprotein L domain-containing protein 1 (APOLD1) Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 10xHis-tagged, 33.4kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Apolipoprotein L domain-containing protein 1 (APOLD1) Protein

Recombinant Human Apolipoprotein L domain-containing protein 1 (APOLD1) Protein — Is a modulator of endothelial barrier permeability, required for proper organization of endothelial cell-cell junctions and cytoskeleton. Purity >85%.

SKU: BCRECP-00089TM Category:
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Product Specifications

Uniprot No.Q96LR9
Gene NamesAPOLD1
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-279aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight33.4kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Is a modulator of endothelial barrier permeability, required for proper organization of endothelial cell-cell junctions and cytoskeleton. It also plays a role in the modulation of secretory autophagy. May affect blood-brain barrier permeability.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Cell junction; Cytoplasmic vesicle, secretory vesicle
Tissue Specificity: Expressed in neonatal dermal microvascular endothelial cells
Disease Association: Bleeding disorder, vascular-type (BDVAS) : An autosomal dominant disorder characterized by increased bleeding tendency, without platelet dysfunction. Affected individuals experience spontaneous episodic bleeding, usually beginning in childhood. Clinical manifestations include epistaxis, oral cavity bleeding, menorrhagia, and excessive bleeding during surgery or childbirth. [The disease may be caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Apolipoprotein L domain-containing protein 1 (APOLD1) Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 1-279aa, with N-terminal 10xHis-tagged tag, molecular weight 33.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Is a modulator of endothelial barrier permeability, required for proper organization of endothelial cell-cell junctions and cytoskeleton (PubMed:35638551). It also plays a role in the modulation of secretory autophagy (PubMed:35638551). May affect blood-brain barrier permeability

Subcellular Location

Cell membrane (Multi-pass membrane protein); Cell junction; Cytoplasmic vesicle, secretory vesicle

Disease Association

Bleeding disorder, vascular-type (BDVAS) : An autosomal dominant disorder characterized by increased bleeding tendency, without platelet dysfunction. Affected individuals experience spontaneous episodic bleeding, usually beginning in childhood. Clinical manifestations include epistaxis, oral cavity bleeding, menorrhagia, and excessive bleeding during surgery or childbirth. [The disease may be caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in neonatal dermal microvascular endothelial cells

Gene: APOLD1  |  Organism: Homo sapiens  |  Synonyms: Vascular early response gene protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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