Recombinant Human Aquaporin-2 (AQP2) Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 10xHis-tagged, 30.3kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Aquaporin-2 (AQP2) Protein

Recombinant Human Aquaporin-2 (AQP2) Protein — Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Purity >85%.

SKU: BCRECP-00292TM Category:
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Product Specifications

Uniprot No.P41181
Gene NamesAQP2
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-271aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight30.3kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis. Could also be permeable to glycerol.

Biological Context

Subcellular Location: Apical cell membrane (Multi-pass membrane protein); Basolateral cell membrane (Multi-pass membrane protein); Cell membrane (Multi-pass membrane protein); Cytoplasmic vesicle membrane (Multi-pass membrane protein); Golgi apparatus, trans-Golgi network membrane (Multi-pass membrane protein)
Tissue Specificity: Expressed in collecting tubules in kidney medulla (at protein level). Detected in kidney
Disease Association: Diabetes insipidus, nephrogenic, 2, autosomal (NDI2) : A disorder caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. Characterized by excessive water drinking (polydipsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Inheritance can be autosomal dominant or recessive. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Aquaporin-2 (AQP2) Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 1-271aa, with N-terminal 10xHis-tagged tag, molecular weight 30.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed:15509592, PubMed:7510718, PubMed:7524315, PubMed:8140421, PubMed:8584435). Plays an essential role in renal water homeostasis (PubMed:15509592, PubMed:7524315, PubMed:8140421). Could also be permeable to glycerol (PubMed:8584435)

Subcellular Location

Apical cell membrane (Multi-pass membrane protein); Basolateral cell membrane (Multi-pass membrane protein); Cell membrane (Multi-pass membrane protein); Cytoplasmic vesicle membrane (Multi-pass membrane protein); Golgi apparatus, trans-Golgi network membrane (Multi-pass membrane protein)

Disease Association

Diabetes insipidus, nephrogenic, 2, autosomal (NDI2) : A disorder caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. Characterized by excessive water drinking (polydipsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Inheritance can be autosomal dominant or recessive. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in collecting tubules in kidney medulla (at protein level) (PubMed:7510718). Detected in kidney (PubMed:7510718)

Subunit

Homotetramer (PubMed:24733887). Interacts with micropeptide MIAC; the interaction leads to a reduction of filamentous actin fibers and inhibition of the EREG/EGFR signaling pathway (PubMed:32176498, PubMed:36117171)

Gene: AQP2  |  Organism: Homo sapiens  |  Synonyms: ADH water channel; Aquaporin-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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