Recombinant Human ATP-dependent DNA helicase Q5 (RECQL5), partial, Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 26.1kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human ATP-dependent DNA helicase Q5 (RECQL5), partial

Recombinant Human ATP-dependent DNA helicase Q5 (RECQL5), partial — DNA helicase that plays an important role in DNA replication, transcription and repair. Purity >95%.

SKU: BCRECP-001529 Category:
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Product Specifications

Product SkuBCRECP-001529
Product DescriptionRecombinant Human ATP-dependent DNA helicase Q5 (RECQL5) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 39-213aa. [Accession | O94762].
Uniprot No.O94762
Gene NamesRECQL5
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region39-213aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight26.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

DNA helicase that plays an important role in DNA replication, transcription and repair. Probably unwinds DNA in a 3'-5' direction (Probable). Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription-associated genomic instability.

Biological Context

Subcellular Location: Cytoplasm
Tissue Specificity: Ubiquitous

Product Specifications

Recombinant Human ATP-dependent DNA helicase Q5 (RECQL5), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 39-213aa, with C-terminal 6xHis-tagged tag, molecular weight 26.1kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

DNA helicase that plays an important role in DNA replication, transcription and repair (PubMed:20643585, PubMed:22973052, PubMed:28100692). Probably unwinds DNA in a 3'-5' direction (Probable) (PubMed:28100692). Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription-associated genomic instability (PubMed:20231364). Also associates with POLR1A and enforces the stability of ribosomal DNA arrays (PubMed:27502483). Plays an important role in mitotic chromosome separation after cross-over events and cell cycle progress (PubMed:22013166). Mechanistically, removes RAD51 filaments protecting stalled replication forks at common fragile sites and stimulates MUS81-EME1 endonuclease leading to mitotic DNA synthesis (PubMed:28575661). Required for efficient DNA repair, including repair of inter-strand cross-links (PubMed:23715498). Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination. A core helicase fragment (residues 11-609) binds preferentially to splayed duplex, looped and ssDNA (PubMed:28100692)

Subcellular Location

Cytoplasm

Tissue Specificity

Ubiquitous

Subunit

Monomer. Interacts with TOP2A, TOP3A and TOP3B (PubMed:10710432, PubMed:20231364). Isoform beta interacts with RNA polymerase II subunit POLR2A (PubMed:20231364, PubMed:20348101, PubMed:23748380). Identified in a complex with the RNA polymerase II core bound to DNA (PubMed:20231364, PubMed:20348101). Interacts (via C-terminus) with POLR1A (PubMed:27502483). Isoform beta interacts with RAD51 (PubMed:20231364, PubMed:20348101). Interacts with WRN; this interaction stimulates WRN helicase activity on DNA fork duplexes (PubMed:23180761). Interacts with MUS1; this interaction promotes MUS81-dependent mitotic DNA synthesis (PubMed:28575661)

Gene: RECQL5  |  Organism: Homo sapiens  |  Synonyms: DNA 3'-5' helicase RecQ5; DNA helicase, RecQ-like type 5; RecQ protein-like 5
Key Publications

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Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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