Recombinant Human ATP-dependent RNA helicase DDX19B (DDX19B) (Active), Homo sapiens (Human), E.coli, Tag type will be determined during the manufacturing process. The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially., 80.8 kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human ATP-dependent RNA helicase DDX19B (DDX19B) (Active)

Recombinant Human ATP-dependent RNA helicase DDX19B (DDX19B) (Active) — ATP-dependent RNA helicase involved in mRNA export from the nucleus. Purity >85%.

SKU: BCACP-000044 Category:
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Product SKUBCACP-000044
Product DescriptionRecombinant Human ATP-dependent RNA helicase DDX19B (DDX19B) protein is expressed from E.coli with Tag type will be determined during the manufacturing process. The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.. It contains 2-479aa. [Accession | Q9UMR2].
Uniprot No.Q9UMR2
Gene NamesDDX19B
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-479aa
SpeciesHomo sapiens (Human)
Tag InfoTag type will be determined during the manufacturing process. The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Molecular weight80.8 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized ACKR2 at 1 μg/ml can bind human CCL2, the EC50 of human CCL2 protein is 23.52-30.99 μg/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesATP-dependent RNA helicase DDX19B; DBP5; Dbp5; yeast; homolog of; DD19B_HUMAN; DDX19; DDX19B; DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B; DEAD box protein 19B; DEAD box RNA helicase DEAD5; DEAD-box protein 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5; yeast; homolog); DEAD/H BOX 19; helicase DEAD5; RNAh; TDBP; yeast Dbp5 homolog

Function

ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins.

Biological Context

Subcellular Location: Cytoplasm; Nucleus, nucleoplasm

Product Specifications

Recombinant Human ATP-dependent RNA helicase DDX19B (DDX19B) (Active) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-479aa, with Tag type will be determined during the manufacturing process. The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. tag, molecular weight 80.8 kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized ACKR2 at 1 μg/ml can bind human CCL2, the EC50 of human CCL2 protein is 23.52-30.99 μg/ml.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

ATP-dependent RNA helicase involved in mRNA export from the nucleus (PubMed:10428971). Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins (PubMed:10428971)

Subcellular Location

Cytoplasm; Nucleus, nucleoplasm

Subunit

Associates with the nuclear pore complex via interaction with NUP214 (PubMed:10428971, PubMed:19208808, PubMed:19219046). Interacts with NUP214 or RNA in a mutually exclusive manner (PubMed:19208808, PubMed:19219046, PubMed:19244245)

Gene: DDX19B  |  Organism: Homo sapiens  |  Synonyms: DEAD box RNA helicase DEAD5; DEAD box protein 19B
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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