Recombinant Human ATP-dependent RNA helicase DDX19B (DDX19B) (Active), Homo sapiens (Human), E.coli, Tag type will be determined during the manufacturing process. The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially., 80.8 kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human ATP-dependent RNA helicase DDX19B (DDX19B) (Active)

Recombinant Human ATP-dependent RNA helicase DDX19B (DDX19B) (Active) — ATP-dependent RNA helicase involved in mRNA export from the nucleus. Purity >85%.

SKU: BCACP-000044 Category:
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Product SKUBCACP-000044
Product DescriptionRecombinant Human ATP-dependent RNA helicase DDX19B (DDX19B) protein is expressed from E.coli with Tag type will be determined during the manufacturing process. The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.. It contains 2-479aa. [Accession | Q9UMR2].
Uniprot No.Q9UMR2
Gene NamesDDX19B
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-479aa
SpeciesHomo sapiens (Human)
Tag InfoTag type will be determined during the manufacturing process. The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Molecular weight80.8 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized ACKR2 at 1 μg/ml can bind human CCL2, the EC50 of human CCL2 protein is 23.52-30.99 μg/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesATP-dependent RNA helicase DDX19B; DBP5; Dbp5; yeast; homolog of; DD19B_HUMAN; DDX19; DDX19B; DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B; DEAD box protein 19B; DEAD box RNA helicase DEAD5; DEAD-box protein 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5; yeast; homolog); DEAD/H BOX 19; helicase DEAD5; RNAh; TDBP; yeast Dbp5 homolog

Function

ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins.

Biological Context

Subcellular Location: Cytoplasm; Nucleus, nucleoplasm

Product Specifications

Recombinant Human ATP-dependent RNA helicase DDX19B (DDX19B) (Active) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-479aa, with Tag type will be determined during the manufacturing process. The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. tag, molecular weight 80.8 kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

ATP-dependent RNA helicase involved in mRNA export from the nucleus (PubMed:10428971). Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins (PubMed:10428971)

Subcellular Location

Cytoplasm; Nucleus, nucleoplasm

Subunit

Associates with the nuclear pore complex via interaction with NUP214 (PubMed:10428971, PubMed:19208808, PubMed:19219046). Interacts with NUP214 or RNA in a mutually exclusive manner (PubMed:19208808, PubMed:19219046, PubMed:19244245)

Gene: DDX19B  |  Organism: Homo sapiens  |  Synonyms: DEAD box RNA helicase DEAD5; DEAD box protein 19B
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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