Recombinant Human Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT2), Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 57.3kDa, purity >90% as determined by SDS-PAGE.
,

Recombinant Human Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT2)

Recombinant Human Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT2) — Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. Purity >90%.

SKU: BCRECP-000153 Categories: ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-000153
Product DescriptionRecombinant Human Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT2) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 28-392aa. [Accession | O15382].
Uniprot No.O15382
Gene NamesBCAT2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region28-392aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight57.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. Branched chain amino acid catabolism plays a role in adipocyte differentiation by providing lipogenic acetyl-CoA pools in differentiated adipocytes. Mechanistically, acetyl-CoA derived from branched chain amino acid catabolism is used by EP300/p300 to acetylate and inhibit PRDM16, thereby preventing adipose tissue browning.

Biological Context

Subcellular Location: Mitochondrion
Tissue Specificity: Ubiquitous
Disease Association: Hypervalinemia and hyperleucine-isoleucinemia (HVLI) : An autosomal recessive metabolic disorder characterized by highly elevated plasma concentrations of valine and leucine/isoleucine. Affected individuals suffer from headache and mild memory impairment. [The disease is caused by variants affecting the gene represented in this entry. A patient with hypervalinemia and hyperleucine-isoleucinemia was identified as compound heterozygote for Gln-170 (inherited from his father) and Lys-264 (inherited from his mother), both variants reduced the catalytic activity of the enzyme. After treatment with vitamin B6, a precursor of pyridoxal 5'-phosphate, a BCAT2 cofactor, the blood levels of branched chain amino acids, especially valine, were decreased and brain lesions were improved]

Product Specifications

Recombinant Human Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT2) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 28-392aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 57.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine (PubMed:17050531, PubMed:25653144, PubMed:8702755). Branched chain amino acid catabolism plays a role in adipocyte differentiation by providing lipogenic acetyl-CoA pools in differentiated adipocytes (By similarity). Mechanistically, acetyl-CoA derived from branched chain amino acid catabolism is used by EP300/p300 to acetylate and inhibit PRDM16, thereby preventing adipose tissue browning (By similarity). May also function as a transporter of branched chain alpha-keto acids (By similarity)

Subcellular Location

Mitochondrion

Disease Association

Hypervalinemia and hyperleucine-isoleucinemia (HVLI) : An autosomal recessive metabolic disorder characterized by highly elevated plasma concentrations of valine and leucine/isoleucine. Affected individuals suffer from headache and mild memory impairment. [The disease is caused by variants affecting the gene represented in this entry. A patient with hypervalinemia and hyperleucine-isoleucinemia was identified as compound heterozygote for Gln-170 (inherited from his father) and Lys-264 (inherited from his mother), both variants reduced the catalytic activity of the enzyme. After treatment with vitamin B6, a precursor of pyridoxal 5'-phosphate, a BCAT2 cofactor, the blood levels of branched chain amino acids, especially valine, were decreased and brain lesions were improved]

Tissue Specificity

Ubiquitous

Subunit

Homodimer

Gene: BCAT2  |  Organism: Homo sapiens  |  Synonyms: Placental protein 18
Key Publications

Explore closely related product collections, technology platforms, and custom production resources.

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

Shopping Cart
Scroll to Top