Recombinant Human C-C motif chemokine 2 protein (CCL2), Homo sapiens (Human), E.Coli, Tag-Free, 8.7 kDa, purity >>96% as determined by SDS-PAGE.
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Recombinant Human C-C motif chemokine 2 protein (CCL2)

Recombinant Human C-C motif chemokine 2 protein (CCL2) — Acts as a ligand for C-C chemokine receptor CCR2. Purity >96%.

SKU: BCACP-000758 Categories: ,
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Product SKUBCACP-000758
Product DescriptionRecombinant Human C-C motif chemokine 2 protein (CCL2) protein is expressed from E.Coli with Tag-Free. It contains 24-99aa. [Accession | P13500].
Uniprot No.P13500
Gene NamesCCL2
Purity>96% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region24-99aa
SpeciesHomo sapiens (Human)
Tag InfoTag-Free
Molecular weight8.7 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/ml, corresponding to a specific activity of >5.0x105 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesC-C motif chemokine 2; CCL2; CCL2_HUMAN; Chemokine (C C motif) ligand 2; GDCF 2; GDCF-2; GDCF2; HC11; HSMCR30; JE; MCAF; MCP 1; MCP-1; MCP1; MGC9434; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2 (monocyte chemotactic protein 1; homologous to mouse Sig je); Small inducible cytokine A2; Small inducible cytokine subfamily A (Cys Cys); member 2; Small-inducible cytokine A2; SMC CF; SMC-CF; SMCCF

Function

Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level). Expressed in monocytes

Product Specifications

Recombinant Human C-C motif chemokine 2 protein (CCL2) is a recombinant protein from Homo sapiens (Human), expressed in E.Coli, covering amino acids 24-99aa, with Tag-Free tag, molecular weight 8.7 kDa, purity >96% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:10529171, PubMed:10587439, PubMed:9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:10587439, PubMed:9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8195247, PubMed:8627182, PubMed:9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690)

Subcellular Location

Secreted

Tissue Specificity

Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level) (PubMed:23765988). Expressed in monocytes (PubMed:2513477)

Subunit

Monomer or homodimer; in equilibrium (PubMed:15033992, PubMed:8898111, PubMed:8989326, PubMed:9837883). Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans (PubMed:9792674). Interacts with TNFAIP6 (via Link domain)

Gene: CCL2  |  Organism: Homo sapiens  |  Synonyms: HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Small-inducible cytokine A2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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