Recombinant Human C-C motif chemokine 25 protein (CCL25), Homo sapiens (Human), E.Coli, Tag-Free, 14.3 kDa, purity >>97% as determined by SDS-PAGE.
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Recombinant Human C-C motif chemokine 25 protein (CCL25)

Recombinant Human C-C motif chemokine 25 protein (CCL25) — Potentially involved in T-cell development. Purity >97%.

SKU: BCACP-000774 Categories: ,
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Product SKUBCACP-000774
Product DescriptionRecombinant Human C-C motif chemokine 25 protein (CCL25) protein is expressed from E.Coli with Tag-Free. It contains 24-150aa. [Accession | O15444].
Uniprot No.O15444
Gene NamesCCL25
Purity>97% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region24-150aa
SpeciesHomo sapiens (Human)
Tag InfoTag-Free
Molecular weight14.3 kDa
ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 1.0-10.0 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesA130072A22Rik; C-C motif chemokine 25; CCL 25; CCL25; CCL25_HUMAN; Chemokine (C C motif) ligand 25; Chemokine (CC motif) ligand 25; Chemokine C C motif ligand 25; Chemokine CC motif ligand 25; Chemokine TECK; Ck beta 15; Ckb 15; Ckb15; MGC125074; MGC150327; SCY A25; SCYA 25; SCYA25; Small inducible cytokine A25; Small inducible cytokine A25 isoform 1 precursor; Small inducible cytokine A25 isoform 2 precursor; Small inducible cytokine subfamily A (Cys Cys) member 25; Small inducible cytokine subfamily A Cys Cys member 25; Small inducible cytokine subfamily A; member 25; Small-inducible cytokine A25; TECK; TECKvar; Thymus expressed chemokine; Thymus-expressed chemokine

Function

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine

Product Specifications

Recombinant Human C-C motif chemokine 25 protein (CCL25) is a recombinant protein from Homo sapiens (Human), expressed in E.Coli, covering amino acids 24-150aa, with Tag-Free tag, molecular weight 14.3 kDa, purity >97% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4

Subcellular Location

Secreted

Tissue Specificity

Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine

Gene: CCL25  |  Organism: Homo sapiens  |  Synonyms: Chemokine TECK; Small-inducible cytokine A25; Thymus-expressed chemokine
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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