Recombinant Human C-Jun-amino-terminal kinase-interacting protein 4 (SPAG9), partial, Homo sapiens (Human), Baculovirus, C-terminal 6xHis-tagged, 15.8kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human C-Jun-amino-terminal kinase-interacting protein 4 (SPAG9), partial

Recombinant Human C-Jun-amino-terminal kinase-interacting protein 4 (SPAG9), partial — The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Purity >95%.

SKU: BCRECP-001686 Categories: , ,
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Product Specifications

Product SkuBCRECP-001686
Product DescriptionRecombinant Human C-Jun-amino-terminal kinase-interacting protein 4 (SPAG9) Protein is expressed from Baculovirus with C-terminal 6xHis-tagged. It contains 408-534aa. [Accession | O60271].
Uniprot No.O60271
Gene NamesSPAG9
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region408-534aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight15.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasDevelopmental Biology

Function

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex. Assists PIKFYVE selective functionality in microtubule-based endosome-to-TGN trafficking.

Biological Context

Subcellular Location: Cytoplasmic vesicle, secretory vesicle, acrosome
Tissue Specificity: Expressed in testis

Product Specifications

Recombinant Human C-Jun-amino-terminal kinase-interacting protein 4 (SPAG9), partial is a recombinant protein from Homo sapiens (Human), expressed in Baculovirus, covering amino acids 408-534aa, with C-terminal 6xHis-tagged tag, molecular weight 15.8kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed:14743216). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex (PubMed:29146937). Assists PIKFYVE selective functionality in microtubule-based endosome-to-TGN trafficking (By similarity)

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, acrosome

Tissue Specificity

Expressed in testis

Subunit

Interacts with MAPK8, MAPK9, MAPK10

Gene: SPAG9  |  Organism: Homo sapiens  |  Synonyms: Cancer/testis antigen 89; Human lung cancer oncogene 6 protein; JNK-associated leucine-zipper protein; Mitogen-activated protein kinase 8-interacting protein 4; Proliferation-inducing protein 6; Protein highly expressed in testis; Sperm surface protein; Sperm-associated antigen 9; Sperm-specific protein; Sunday driver 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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