Recombinant Human C5a anaphylatoxin chemotactic receptor 1 (C5AR1)-VLPs (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions), 41.1 kDa
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Recombinant Human C5a anaphylatoxin chemotactic receptor 1 (C5AR1)-VLPs (Active)

Recombinant Human C5a anaphylatoxin chemotactic receptor 1 (C5AR1)-VLPs (Active) — Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a, stimulating chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.

SKU: BCACP-000163 Categories: ,
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Product SKUBCACP-000163
Product DescriptionRecombinant Human C5a anaphylatoxin chemotactic receptor 1 (C5AR1)-VLPs protein is expressed from Mammalian cell with C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions). It contains 1-350aa. [Accession | P21730].
Uniprot No.P21730
Gene NamesC5AR1
Expression SystemMammalian cell
Expression Region1-350aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Molecular weight41.1 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CCR5 at 10 μg/mL can bind Anti-CCR5 recombinant antibody . The EC50 is 1.099-1.287 ng/mL.The VLPs is negative control.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesC5AR1; C5AR; C5R1; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR; CD antigen CD88

Function

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a, stimulating chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. C5AR1 is coupled to G(i)/G(o) (GNAI1 or GNAO1) G alpha proteins and mediates inhibition of adenylate cyclase.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Cytoplasmic vesicle

Product Specifications

Recombinant Human C5a anaphylatoxin chemotactic receptor 1 (C5AR1)-VLPs (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 1-350aa, with C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions) tag, molecular weight 41.1 kDa. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a, stimulating chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production (PubMed:10636859, PubMed:15153520, PubMed:1847994, PubMed:29300009, PubMed:36806352, PubMed:7622471, PubMed:8182049, PubMed:9553099). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed:36806352, PubMed:37852260). C5AR1 is coupled to G(i)/G(o) (GNAI1 or GNAO1) G alpha proteins and mediates inhibition of adenylate cyclase (PubMed:36806352, PubMed:37852260). The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events (PubMed:7622471, PubMed:8182049, PubMed:9553099)

Subcellular Location

Cell membrane (Multi-pass membrane protein); Cytoplasmic vesicle

Subunit

(Microbial infection) Interacts (via N-terminal domain) with S.aureus chemotaxis inhibitory protein (CHIPS); the interaction blocks the receptor and may thus inhibit the immune response

Gene: C5AR1  |  Organism: Homo sapiens  |  Synonyms: C5a anaphylatoxin chemotactic receptor
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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