Recombinant Human Carbonic anhydrase 12 (CA12), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 47.1kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Carbonic anhydrase 12 (CA12), partial

Recombinant Human Carbonic anhydrase 12 (CA12), partial — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >90%.

SKU: BCRECP-000201 Category:
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Product Specifications

Product SkuBCRECP-000201
Product DescriptionRecombinant Human Carbonic anhydrase 12 (CA12) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 25-301aa. [Accession | O43570].
Uniprot No.O43570
Gene NamesCA12
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region25-301aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight47.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Reversible hydration of carbon dioxide.

Biological Context

Subcellular Location: Membrane (Single-pass type I membrane protein); Cell membrane
Tissue Specificity: Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium
Disease Association: Hyperchlorhidrosis, isolated (HYCHL) : An autosomal recessive disorder characterized by excessive sweating and increased sweat chloride levels. Affected individuals suffer from episodes of hyponatremic dehydration and report increased amounts of visible salt precipitates in sweat. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Carbonic anhydrase 12 (CA12), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 25-301aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 47.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Reversible hydration of carbon dioxide

Subcellular Location

Membrane (Single-pass type I membrane protein); Cell membrane

Disease Association

Hyperchlorhidrosis, isolated (HYCHL) : An autosomal recessive disorder characterized by excessive sweating and increased sweat chloride levels. Affected individuals suffer from episodes of hyponatremic dehydration and report increased amounts of visible salt precipitates in sweat. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium (PubMed:26911677)

Subunit

Homodimer

Gene: CA12  |  Organism: Homo sapiens  |  Synonyms: Carbonate dehydratase XII; Carbonic anhydrase XII; Tumor antigen HOM-RCC-3.1.3
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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