Recombinant Human Carbonic anhydrase 5A, mitochondrial (CA5A), Homo sapiens (Human), Mammalian cell, Tag-Free, 30.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Carbonic anhydrase 5A, mitochondrial (CA5A)

Recombinant Human Carbonic anhydrase 5A, mitochondrial (CA5A) — Mitochondrial carbonic anhydrase that catalyzes the reversible conversion of carbon dioxide to bicarbonate/HCO3. Purity >85%.

SKU: BCRECP-000205 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-000205
Product DescriptionRecombinant Human Carbonic anhydrase 5A, mitochondrial (CA5A) Protein is expressed from Mammalian cell with Tag-Free. It contains 39-305aa. [Accession | P35218].
Uniprot No.P35218
Gene NamesCA5A
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region39-305aa
SpeciesHomo sapiens (Human)
Tag InfoTag-Free
Molecular weight30.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Mitochondrial carbonic anhydrase that catalyzes the reversible conversion of carbon dioxide to bicarbonate/HCO3. Mitochondria are impermeable to HCO3, and thus this intramitochondrial carbonic anhydrase is pivotal in providing HCO3 for multiple mitochondrial enzymes that catalyze the formation of essential metabolites of intermediary metabolism in the urea and Krebs cycles.

Biological Context

Subcellular Location: Mitochondrion
Disease Association: Hyperammonemia due to carbonic anhydrase VA deficiency (CA5AD) : An autosomal recessive inborn error of metabolism, clinically characterized by infantile hyperammonemic encephalopathy. Metabolic abnormalities include hypoglycemia, hyperlactatemia, metabolic acidosis and respiratory alkalosis. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Carbonic anhydrase 5A, mitochondrial (CA5A) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 39-305aa, with Tag-Free tag, molecular weight 30.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Mitochondrial carbonic anhydrase that catalyzes the reversible conversion of carbon dioxide to bicarbonate/HCO3 (PubMed:24530203, PubMed:8356065). Mitochondria are impermeable to HCO3, and thus this intramitochondrial carbonic anhydrase is pivotal in providing HCO3 for multiple mitochondrial enzymes that catalyze the formation of essential metabolites of intermediary metabolism in the urea and Krebs cycles (PubMed:24530203)

Subcellular Location

Mitochondrion

Disease Association

Hyperammonemia due to carbonic anhydrase VA deficiency (CA5AD) : An autosomal recessive inborn error of metabolism, clinically characterized by infantile hyperammonemic encephalopathy. Metabolic abnormalities include hypoglycemia, hyperlactatemia, metabolic acidosis and respiratory alkalosis. [The disease is caused by variants affecting the gene represented in this entry]

Gene: CA5A  |  Organism: Homo sapiens  |  Synonyms: Carbonate dehydratase VA; Carbonic anhydrase VA
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top