Recombinant Human Carboxypeptidase N catalytic chain (CPN1), Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 57.5kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human Carboxypeptidase N catalytic chain (CPN1)

Recombinant Human Carboxypeptidase N catalytic chain (CPN1) — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >85%.

SKU: BCRECP-000417 Categories: , ,
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Product Specifications

Product SkuBCRECP-000417
Product DescriptionRecombinant Human Carboxypeptidase N catalytic chain (CPN1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 21-458aa. [Accession | P15169].
Uniprot No.P15169
Gene NamesCPN1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region21-458aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight57.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.

Biological Context

Subcellular Location: Secreted, extracellular space
Tissue Specificity: Synthesized in the liver and secreted in plasma
Disease Association: Carboxypeptidase N deficiency (CPND) : An autosomal recessive disorder characterized by angioedema, acute or chronic urticaria, and hay fever or asthma. Affected individuals have levels of carboxypeptidase N below the reference range. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Carboxypeptidase N catalytic chain (CPN1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 21-458aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 57.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation

Subcellular Location

Secreted, extracellular space

Disease Association

Carboxypeptidase N deficiency (CPND) : An autosomal recessive disorder characterized by angioedema, acute or chronic urticaria, and hay fever or asthma. Affected individuals have levels of carboxypeptidase N below the reference range. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Synthesized in the liver and secreted in plasma

Subunit

Tetramer of two catalytic chains and two glycosylated inactive chains

Gene: CPN1  |  Organism: Homo sapiens  |  Synonyms: Anaphylatoxin inactivator; Arginine carboxypeptidase; Carboxypeptidase N polypeptide 1; Carboxypeptidase N small subunit; Kininase-1; Lysine carboxypeptidase; Plasma carboxypeptidase B; Serum carboxypeptidase N
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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