Recombinant Human Casein kinase I isoform epsilon (CSNK1E), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 51.3kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Casein kinase I isoform epsilon (CSNK1E)

Recombinant Human Casein kinase I isoform epsilon (CSNK1E) — Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (Probable). Purity >90%.

SKU: BCRECP-000441 Categories: , ,
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Product Specifications

Product SkuBCRECP-000441
Product DescriptionRecombinant Human Casein kinase I isoform epsilon (CSNK1E) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-416aa. [Accession | P49674].
Uniprot No.P49674
Gene NamesCSNK1E
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-416aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight51.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (Probable). Participates in Wnt signaling. Phosphorylates DVL1.

Biological Context

Subcellular Location: Cytoplasm; Nucleus
Tissue Specificity: Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes

Product Specifications

Recombinant Human Casein kinase I isoform epsilon (CSNK1E) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-416aa, with N-terminal 6xHis-tagged tag, molecular weight 51.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (Probable). Participates in Wnt signaling (PubMed:12556519, PubMed:23413191). Phosphorylates DVL1 (PubMed:12556519). Phosphorylates DVL2 (PubMed:23413191). Phosphorylates NEDD9/HEF1 (By similarity). Central component of the circadian clock (PubMed:16790549). In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation (PubMed:15917222, PubMed:16790549). Controls PER1 and PER2 nuclear transport and degradation (By similarity). Inhibits cytokine-induced granuloytic differentiation (PubMed:15070676)

Subcellular Location

Cytoplasm; Nucleus

Tissue Specificity

Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes

Subunit

Monomer (PubMed:23106386). Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, ARTNL/BMAL1 or ARTNL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins (By similarity). Interacts with PER1 (PubMed:10790862). Interacts with ANKRD6 (By similarity). Interacts with DBNDD2 (PubMed:16618118). Interacts with LRP5 and LRP6 (PubMed:16513652). Interacts with SOCS3 (PubMed:15070676). Interacts with SNAI1 (via zinc fingers) (PubMed:20305697). Interacts with DDX3X; this interaction greatly enhances CSNK1E affinity for ATP and DVL2 phosphorylation, but inhibits DDX3X ATPase/helicase activity. In the presence of RNA, the interaction is decreased (PubMed:23413191, PubMed:29222110). Interacts with FAM83A, FAM83B, FAM83E and FAM83H (via DUF1669) (PubMed:29789297)

Gene: CSNK1E  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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